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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
1mg/500μg/50μg/10μg
| 规格： | 1mg | 产品价格： | ￥25680.0 |
|---|---|---|---|
| 规格： | 500μg | 产品价格： | ￥17480.0 |
| 规格： | 50μg | 产品价格： | ￥2580.0 |
| 规格： | 10μg | 产品价格： | ￥980.0 |
产品信息
| 别名 | FGFL |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MHPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVG,S.SDPLSMVGPSQGRSPSYAS with polyhistidine tag at the C-terminus. |
| 检索号 | Q9NSA1.1 |
| 分子量 | 20.35 kDa |
| 表观分子量 | 25 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce proliferation in BaF3 cells transfected with human FGFRIIIc. The ED50 for this effect is <0.4 μg/mL. |
产品特性
| 纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution,store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
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文献和实验浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
-19]； xChIP：用来研究DNA及低结合力蛋白，采用甲醛或紫外线进行 DNA 和蛋白交联，超声波片段化染色质，然后进行片段富集及后续分析，适用于多数非组蛋白的蛋白，例如 [9, 15, 20]。 X-ChIP 试验的一般过程 以上两种方法在分离 DNA-蛋白质复合物之后，对 DNA 进行 PCR 扩增，验证目标序列的存在。除验证实验外，ChIP DNA也可以进行测序分析，这种方法被称为 ChIP-seq [22] ；也可做芯片分析，这种方法被称为 ChIP on CHIP 或 ChIP
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
(approximately 1.0 x 106 cells/60 mm plate) on the Cultrex BME coated plate in Conditioned Media containing 4 ng/mL of recombinant human FGF basic. Change the media daily. Monitor the cells for the desired confluency. Figure
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
at 200 x g for 4 minutes. Remove the supernatant and gently flick the pellet. Resuspend the pellet in an appropriate amount of Human Feeder Cell Conditioned Medium supplemented with 4 ng/mL of recombinant human FGF basic. Add
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