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- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
Avidin
- 库存:
999
- 供应商:
上海淳麦生物
- 规格:
/5ug/20ug/1mg
Avidin
英文简称:
Avidin
中文名称:
抗生物素蛋白
背景资料:
Avidin is a tetrameric protein of 4 identical subunits (homotetramer) each of which can bind to biotin with a high degrAvidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containg 4 subunits each with a binding site for biotin. The Avidin is purified by a鸡蛋白中提取纯化inity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB). of a鸡蛋白中提取纯化inity and specificity. Avidin molecular weight in its tetrameric form is estimated to be betwAvidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containg 4 subunits each with a binding site for biotin. The Avidin is purified by a鸡蛋白中提取纯化inity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB).n 66-69 kDa. Avidin is produced in the oviducts of birds, reptiles and amphibians and is subsequently deposited in the whites of their es. In the chicken e white, avidin makes up roughly 0.05% of total protein (approximately 1.8 mg per e). 10% of Avidin’s molecular weight is ascribed to carbohydrate content which is composed of four to five mannose and thrAvidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containg 4 subunits each with a binding site for biotin. The Avidin is purified by a鸡蛋白中提取纯化inity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB). N-acetylglucosamine residues. Avidin has at least thrAvidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containg 4 subunits each with a binding site for biotin. The Avidin is purified by a鸡蛋白中提取纯化inity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB). distinctive oligosaccharide structural type which are similar in structure and composition. The dissociation constant (KD) of avidin is approximately 10-15M, making it one of the strongest known non-covalent bonds.
产品描述:
Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containg 4 subunits each with a binding site for biotin. The Avidin is purified by a鸡蛋白中提取纯化inity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB).
来源宿主:
鸡蛋白中提取纯化
溶解建议:
Sterile Filtered lyophilized powder. It is recommended to reconstitute the lyophilized Avidin in sterile 18MΩ-cm H2O not less than 100ug/ml or more than 10mg/ml solutions.
保存建议:
Avidin although stable at 4℃ for 3 weeks, should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
应用领域:
抗生物素蛋白产品可广泛应用于功能研究、蛋白互作研究、免疫检测研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词:
抗生物素蛋白、Avidin、多肽、CMBIO、淳麦生物、科研试剂、生物试剂
其他简介:
CMBIO




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文献和实验存在于卵白中的碱性糖蛋白,因它贪婪地(avidly)与生物素结合,故有此名( avidin)。给动物以生的卵白则产生生物素缺乏症,因为生物素蛋白与生物素结合,使之失活。分子量为 68300,含 4个亚单位。在体外(试管中)对含生物素的酶具有特异的抑制作用。
网络 第一节 抗生物素―生物素免疫细胞化学染色法 一、基本原理 很早以前,人们就注意到给动物饲以大量的鸡蛋白,会引起明显的“维生素H缺乏症”,也称为“蛋白质伤害”。经研究发现,在鸡蛋白中含有一种碱性蛋白,分子量为68000,属于糖蛋白,当时命名为卵白素(Avidin),卵白素具有4个同维生素H(Riotin,又名生物素)亲合力极高的结合
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
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