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- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
PKC-g
- 库存:
999
- 供应商:
上海淳麦生物
- 规格:
/5ug/20ug/1mg
Recombinant Human Protein Kinase C gamma
英文简称:
PKC-g
中文名称:
重组人蛋白激酶C-γ
背景资料:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has bPKC-g Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-676 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC-g is purified by proprietary chromatographic techniques.n demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suest that this kinase may be involved in neuropathic pain development. Defects in this protein have bPKC-g Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-676 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC-g is purified by proprietary chromatographic techniques.n associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
产品描述:
PKC-g Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-676 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC-g is purified by proprietary chromatographic techniques.
来源宿主:
Sf9昆虫细胞中重组表达
溶解建议:
提供的人蛋白激酶C-γ(PKC-g)产品为过滤灭菌的透明溶液;其中0.1mg/ml人蛋白激酶C-γ(PKC-g)产品溶解于10mM Tris,pH 7.4,0.1M NaCl,20%甘油,1mM DTT,0.1mM EDTA,0.2mM PMSF,0.03% Brij-35溶液中.
保存建议:
人蛋白激酶C-γ(PKC-g)溶液可以在4℃冷藏保存2-4周;如果想延长保存时间,建议分装后置于-20℃冻存.请避免反复冻融.
应用领域:
重组人蛋白激酶C-γ产品可广泛应用于信号传导研究、药物靶点研究、酶活性研究、代谢研究、功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词:
重组人蛋白激酶C-γ、Recombinant Human Protein Kinase C gamma、PKC-g、重组蛋白、多肽、受体蛋白、酶类、昆虫细胞表达
其他简介:
CMBIO




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文献和实验蛋白激酶C是G蛋白偶联受体系统中的效应物, 在非活性状态下是水溶性的,游离存在于胞质溶胶中,激活后成为膜结合的酶。蛋白激酶C的激活是脂依赖性的,需要膜脂DAG的存在,同时又是Ca2 依赖性的,需要胞质溶胶中Ca2 浓度的升高。当DAG在质膜中出现时,胞质溶胶中的蛋白激酶C被结合到质膜上,然后在Ca2 的作用下被激活。 同蛋白激酶A一样,蛋白激酶C属于多功能丝氨酸和苏氨酸激酶。蛋白激酶C能激活细胞质中的靶酶参与生化反应的调控, 同时也能作用于细胞核中的转录
人蛋白激酶C (PKC) 酶联免疫 分析 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 蛋白激酶C(PKC) 的含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 人蛋白激酶C ( PKC ) 水平。用纯化的 人蛋白激酶C ( PKC ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 蛋白激酶C ( PKC ),再与 HRP 标记的蛋白激酶 C
第五节 蛋白激酶C 蛋白激酶C(protein kinase C,PKC)是一类Ca 2+ 、磷脂依赖性的蛋白激酶,在跨膜信号传递过程中起着重要作用。PKC通过催化多种蛋白质上Ser/Thr磷酸化,调节多种细胞的代谢、生长、增殖和分化。 表 8-3 哺乳动物组织内的PKC亚类 亚 类 氨基酸残基 分子量(kDa) 激 活 剂 表达组织 A组:经典PKC
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