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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Humanized
- 应用范围:
ELISA, Flow Cyt
- 浓度:
详见COA
- 靶点:
FCRL5
- 抗体英文名:
Anti-FCRL5(cevostamab biosimilar without anti-CD3 portion) mAb
- 抗体名:
Anti-FCRL5(cevostamab biosimilar without anti-CD3 portion) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | FCRL5 |
| Uniprot ID | Q96RD9 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | BFCR-4350-A, BFCR-4350A, RG-6160, RO-7187797 |
| 货期 | In Stock |
| 产品英文名称 | Anti-FCRL5(cevostamab biosimilar without anti-CD3 portion) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | BXMAS1;CD307;CD307e;FCRH5;IRTA2;PRO820 |
| 应用 | ELISA, Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Humanized |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000, Flow Cyt 1:100 |
| 应用范围 | Research use only |

Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human FCRL5 Protein, His Tag (PME100625) can bind Anti-FCRL5(cevostamab biosimilar without anti-CD3 portion) mAb (BME100089) in a linear range of 0.61–39.06 ng/mL.

Figure 2. Flow cytometry analysis with 1 μg/mL Anti-FCRL5 (cevostamab biosimilar) mAb (BME100089) on HEK293 cells transfected with Human FCRL5 protein (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human FCRL5(745-847) Protein, mFc Tag (PME101495) can bind Anti-FCRL5(cevostamab biosimilar without anti-CD3 portion) mAb (BME100089) in a linear range of 0.64–80 ng/mL.

Figure 4. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Cynomolgus FCRL5 Protein, His Tag (PME-C100061) can bind Anti-FCRL5(cevostamab biosimilar without anti-CD3 portion) mAb (BME100089) in a linear range of 3.2–16 ng/mL.
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文献和实验- 3) 及 CD45 -PE (HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞以 DAPI 染色先行排除,左右分别为分选前后的细胞群分布。 总结: 几次实验的结果,使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选,平均得率为 84.5%,纯度大于 90%,细胞存活率高于 95%,且目标细胞不含标记,贴近天然,使下游实验更精确。
大学的Andrew Chalmers博士于2014年主导创立的,其号称是世界最大的抗体搜索引擎,该网站上包含了来自204个抗体供应商的近五百万个抗体,汇总了两百多万条抗体引用数据,并且该网站仍在不断更新最新的数据。输入关键词(如:CD3)后点击搜索即可出现所有符合条件的抗体,而该网站的特色是,搜索结果根据抗体被文献引用的次数排序,一般来说,引用越多的产品,质量往往越有保证。 03 Labome 网址:https://www.labome.com/ Labome(www.labome.com
及释放目标细胞。以不具磁性且可逆的试剂,取代一般具高亲和力的完整抗体,从而达到直接由全血或其他样本,进行温和标准化正选的目的。Fab-TACS 正选,化解了传统使用完整抗体进行正选的困境,其对目标细胞无不必要的刺激,避免了分选流程的细胞活化,也避免完整抗体与特定受体的结合而影响的分析准确度。因此,使用 Fab-TACS 无痕正选技术可富集不含标记、未激活的目标细胞,次次精准。人全血 CD3+ T 细胞分选实例1. 所需产品:(1) CD3 Fab-TACS® Gravity Kit, human
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