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10×500 mL/5×500 mL
| 规格: | 10×500 mL | 产品价格: | ¥160.0 |
|---|---|---|---|
| 规格: | 5×500 mL | 产品价格: | ¥80.0 |
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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
Buffer 0.025 M Tris 0.192 M Glycine 0.1% SDS Adjust pH to 8.3 0.2-0.3 ml samples of brain extract, and containing 200 micrograms protein 0.0625 M Tris-HCl (pH 6.8)
from the bottom of the cassette. 3) In one smooth motion, gently pull the comb out of the cassette. 4) Rinse the sample wells with the appropriate 1X SDS Running Buffer. Invert the gel and shake the gel to remove the buffer. Repeat two more times.
for really hydrophobic proteins 1x Running Buffer: 25 mM Tris-HCl 200 mM Glycine 0.1% (w/v) SDS
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