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文献和实验LuMPIS: Luciferase-Based MBP-Pull-Down Protein Interaction Screening System
(eGFP-luc) tags, respectively. Positive protein–protein interactions (PPIs) can be detected after pull-down of the MBP-tagged prey protein using amylose beads followed by the bioluminescence detection of the bound eGFP-luc-tagged bait protein. The LuMPIS
Pull-Down Assays for Plant Hormone Research
Hormonal signals are transduced (and sometimes perceived) by protein–protein interactions. Understanding these interactions is therefore crucial to understanding the network of signalling components as a whole. Often, genetic analysis serves
研究工作者的宝贵时间,QIAGEN推出了Ni-NTA Membrane Protein Kit,专门用于带有His标签的重组膜蛋白的溶解和后续纯化。 Ni-NTA Membrane Protein Kit有两部分组成,一部分是膜蛋白的溶解试剂,该部分包括7种最常用的去污剂,用于供客户筛选最适合自己的去污剂;另一部分是Ni-NTA纯化试剂,用于在膜蛋白溶解用,利用His标签和Ni-NTA的亲和特性进行进一步纯化。该试剂盒最大的优势在于帮助膜蛋白研究者在最短的时间筛选得到最优的去污剂,并且进行Ni-NTA纯化
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