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|---|---|---|---|
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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
Materials Stock Acrylamide: (30%T:0.8%C) 30% by weight of acrylamide 0.8% by weight of N,N'-bis-methylene acrylamide Separation Gel (Final Concentrations) 10% acrylamide
4.1 ), using 15% acrylamide (15%T:5%C) in 2.5 M urea and 0.9 M acetic acid. Set up the gel in the electrophoresis unit and run the gel at 2 mA/gel for 2 hours with no sample, using 0.9 M acetic acid for the running buffer. Dissolve
are not as important, as you are looking for a relatively small number of differences and so can devote more time to the identification of each protein.2D electrophoresis is performed in two steps(1) Isoelectric focusing (IEF) (First dimension electrophoresis): is used
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