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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Antibody Staining of Imaginal Discs
. Monitor reaction under dissecting scope; stop reaction by removing DAB solution and quickly washing tissue 5 times in PBS. Dissect discs and mount in Aqua-mount. ********* Tri-PBS pH
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
to control focus 1) This system uses dual Galilean beam expanders to expand the laser beam to fill the back aperture of the objective (10 mm). Mount the 4 lenses on to carriers and attach them to the rail (L1f1 L2f2 and L3f1' L4f2'). Adjust
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