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文献和实验Measurement with the Light Microscope
the focus. Any ocular scale must be calibrated, using a device called a stage micrometer. A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched
花粉管染色-Aniline Blue Staining of Pollen/Pollen Tubes
Aniline Blue Staining of Pollen/Pollen Tubes花粉管染色 Materials Fixative: 10% Acetic Acid in ethanol 1M NaOH 50 mM KPO4 buffer, pH 7.5: 4.17 mL 1M K2 HPO4 0.83 mL 1M KH2 PO4 0.01% aniline
USE OF THE LIGHT MICROSCOPE Each time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance
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