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文献和实验Tissue Processing and Sectioning
Immerse tissue cassette in 11% formic acid with a stir bar overnight in a fume hood. Rinse in running water for 30- 60 minutes (the smell should be gone). Storage in 70% Ethanol After adequate fixation tissues are transferred to 70% ethanol
This is the most conventional stain for formalin fixed paraffin sections. The hematoxylin stains negatively charged nucleic acids (nuclei and ribosomes) blue. The eosin stains proteins pink. The hematoxylin or the eosin can also be used by themselves in more dilute form
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) upon reaction with free ferric ion. Do not use metal forceps to manipulate the tissue while it is in the X-gal detection buffer. iv. All tissues have endogenous, lysozomal ß-gal. Its pH optimum is very low, and thus it is not very active in the pH
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