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文献和实验Measurement with the Light Microscope
the focus. Any ocular scale must be calibrated, using a device called a stage micrometer. A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched
In Situ Hybridization Using Digoxigenin Labeled Probes
when disposing of unused diaminobenzidine and contaminated materials. 8) Place the slides horizontally in a tray and add 50 µl of staining solution onto each slide, cover with a 22 x 40 mm coverslip and incubate at room temperature for 1 - 10 minutes
A. Plating: To sterilize glass coverslips, dip in ethanol and flame. We use 22x22x1 mm3 coverslips and put them in 6-well plates. Seed 100,000 cells per well overnight and fix the next day. B. Fixation: Remove the media
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