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文献和实验Measurement with the Light Microscope
the focus. Any ocular scale must be calibrated, using a device called a stage micrometer. A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched
Grade Disodiumfluorescein was from Exction, Inc. Low molecular weight (13,000–23,000 MW) poly(vinyl alcohol) (PVA) was from Aldrich. Fluorescein-doped PVA samples were prepared on 1 × 1 in. microscope cover slips, either SIF coated or with SACS prepared
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
incubation Latex gloves Microscope, dissecting Microscope, phase-contrast Pencil with eraser end Rack for holding slides Razor blade Shaking platform Siliconized coverslips (Corning or equivalent; 22 x 22 mm)
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