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文献和实验to a well on a polylysine coated 8-well slide for visualization. The protocol can also easily be adapted for preparing cells for immunofluorescence in microfuge tubes. The protocol is now a seperate document Protocol Annotations refer to notes in list given
Large-scale immunofluorescence
8-well slide9 . Allow cells to settle for 15 minutes or longer (use a humid box to prevent drying out). Gently aspirate off excess solution and immediately add 4 ul of mounting solution to each well. Add a coverslip, seal edges with nail polish and store
to a well on a polylysine coated 8-well slide for visualization. The protocol can also easily be adapted for preparing cells for immunofluorescence in microfuge tubes. The antibody cleanup protocol is now a seperate document Protocol Annotations refer to notes
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