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250 cassettes
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文献和实验Tissue Processing and Sectioning
Immerse tissue cassette in 11% formic acid with a stir bar overnight in a fume hood. Rinse in running water for 30- 60 minutes (the smell should be gone). Storage in 70% Ethanol After adequate fixation tissues are transferred to 70% ethanol
and Dvorak, 1985 ). These untoward changes typically result in compromised antigenicity of many tissue proteins and loss of enzyme specificity. Microwave energy (MWE), used in conjunction with, or as an alternative to, routine chem processing, is gaining
in RPMI must be grown in a 5% CO2 atmosphere. We have one incubator set up for this. If you grow cells in RPMI in 10% CO2, the medium will be too acidic (yellow). DMEM This is an abbreviation for Dulbecco/Vogt modified Eagle's (Harry Eagle) minimal
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