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文献和实验Observation of living and plastic-embedded chick embryos
3- to 6-day chick embryo. Obtain an egg and clean scissors, forceps, a plastic spoon. First prepare a small petri dish with Howard Ringer's solution. Gently rotate egg, clean with 70% ethanol and set down for a few minutes to allow the embryo
Use of HMS Genetics Confocal Microscope
startup • Turn on the scope (switch on base, toward back, on the right). • Turn on the mercury lamp for the scope (small white box to the left of the scope). • Turn on the green laser (far right
In Situ Hybridization Using Digoxigenin Labeled Probes
siliconized slide place two drops of 45% acetic acid and a clean siliconized 18 mm square coverslip. On the coverslip place a small drop (2-3 mm) of fixative( 1/2/3 mixture of lactic acid/water/glacial acetic acid) 3) In the first drop of 45% acetic
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