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文献和实验Measurement with the Light Microscope
the focus. Any ocular scale must be calibrated, using a device called a stage micrometer. A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched
Grade Disodiumfluorescein was from Exction, Inc. Low molecular weight (13,000–23,000 MW) poly(vinyl alcohol) (PVA) was from Aldrich. Fluorescein-doped PVA samples were prepared on 1 × 1 in. microscope cover slips, either SIF coated or with SACS prepared
In Situ Hybridization Using Digoxigenin Labeled Probes
on two 5ml pipettes in a tray lined with moist paper towels. Apply 100µl of a 1:500 dilution of Anti Digoxigenin-POD in 1X PBS, 5mMEDTA. Distribute the solution by placing a 22 x 40 mm coverslip on the slide. 5) Cover the tray with plastic wrap
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