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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 High-Energy Nd:YAG Laser Mirror, 25.4 mm Diameter, 45°, 532 nm Newport 10Q20HE.2 4 SUPREMA Optical Mount, 1.0 inch diameter
volume 19‐G needle (4 ¾ ‐in. long; 1.00 × 120–mm) with Luer‐connection Constant current power supply (300 V and higher) Resealable plastic bags Table 9.8.2 Additional
out a standard (not microinjection) 10 µl micropipette to about 1/5 its starting thickness. Place a stretch of the drawn out part on a 24x50 mm coverslip, and put a drop or two of microinjection oil on top. Mount this on the axiovert, and using the 5X objective
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