- ￥6990
- Sino Biological
- 北京
- 70285-D08H
- 2026年06月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Canine IL1RAP/IL-1RAcP Protein (ECD, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
蛋白名称：Canine IL1RAP/IL-1RAcP Protein (ECD, His Tag)
蛋白构建：A DNA sequence encoding the Canine IL1RAP/IL-1RAcP (A0A8C0NSV9) (Ser21-Glu357) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Ser 21
蛋白分子量：The recombinant Canine IL1RAP/IL-1RAcP comprises 348 amino acids and has a predicted molecular mass of 40.34 kDa. As a result of glycosylation, the band migrates approximately 57.8 kDa in the SDS-PAGE reducing conditions.
蛋白NP号：A0A8C0NSV9
蛋白氨基酸序列：Ser21-Glu357
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文献和实验His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
The Measurement of Monoamine Neurotransmitters in Microdialysis Perfusates Using HPLC-ECD
Microdialysis is a perfusion-based sampling procedure that is now used routinely to study the chemistry of the extracellular environment of a variety of tissues in the living organism (1 ,2 ). A microdialysis probe
Determination of Neurotransmitter Levels in Models of Parkinsons Disease by HPLC-ECD
of reversed-phase HPLC with electrochemical detection (ECD) for simultaneously measuring monoamine neurotransmitters, including dopamine and its metabolites, norepinephrine as well as serotonin and its metabolite. ECD provides an ultrasensitive measurement
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