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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 灵敏度:
0.113 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人整合素β2(ITGb2)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human ITGb2(Integrin Beta 2) Microsample ELISA Kit |
| 别名 | CD18; ITG-B2; LAD; LFA1; MAC1; LCAMB; MF17; LFAb; Complement Component 3 Receptor 3 And 4 Subunit; Lymphocyte Function-Associated Antigen 1; Macrophage Antigen 1 |
| 货号 | ELK4937MS |
| 反应种属 | Human |
| P05107 | P05107 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.113 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGb2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;CD & Adhesion molecule;Tumor immunity; |

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文献和实验正常对照组的血清β2 MG为(1.55±0.30)mg/L。大肠癌组、胃癌组与正常对照组的结果经方差齐性检验和t检验,P 2.2 大肠癌和胃癌手术前后血清β2 MG水平比较大肠癌组和胃癌组术后12 d血清β2 MG水平比术前明显降低(P<0.01),结果见表2。 3 讨论 血、尿中β2 MG在肾脏疾病和肿瘤患者中有不同程度的升高,测定β2 MG的方法有:酶联免疫吸附法(ELISA)、放射免疫法(RIA)、免疫比浊法。ELISA操作繁琐,重复性差,存在准备
and some B cell malignancies Negative staining: normal B cells CD1d Positive staining (normal): thymus (low levels), bowel ELISA KIT & ANTIBODY OF USCNLIFE
要先用4M尿素洗涤一下再用8M尿素溶解.如果不上柱,只是跑跑电泳,可以直接用8M尿素溶解以后,离心取上清,加入适量体积的loading buffer.loading buffer对于包涵体的溶解能力是较弱的. "取200微升菌液,离心后直接加上样buffer,100度3分钟后上样,然后SDSPAGE. 这个方法到底能不能溶解细菌中的包涵体? " 而楼主的问题,虽然loading buffer对于包涵体的溶解能力是较弱的,但是我觉得你的做法只是在鉴定有无表达,用loading buffer
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