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文献和实验Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle. 2. Add 25 ml of CTAB Buffer and transfer to a 50 ml tube. 3. Incubate at 65°C for 20 min with occasional vigorous
(210 ml HCl in 1 L water) . Mix and allow the solution to stand for several hr at room temperature. 3.Add 1 volume of 2.5 N NaOH (100 g NaOH in 1 L water). Mix, and discard the solution. When EtBr conc. is >0.5 µg/ml: * electrophoresis buffer
Decontamination of Ethidium Bromide Solutions and Surfaces
Neutralise with sodium bicarbonate and discard as non-hazardous aqueous waste for solutions. For solids, seal in plastic bags and discard. References: Lunn, G. and Sansone, E.B. (1987) Ethidium bromide: destruction and decontamination
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