Clean-up for Ethidium Bromide
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Caution: EtBr is a powerful mutagen and is moderately toxic. Wear gloves when working.
When EtBr conc. is >0.5 mg/ml:
1.Add sufficient water to reduce the concentration of EtBr to <0.5 mg/ml.
2.Add 1 volume of 0.5 M KMnO4. Mix, add 1 volume of 2.5 N HCl (210 ml HCl in 1 L water) .
Mix and allow the solution to stand for several hr at room temperature.
3.Add 1 volume of 2.5 N NaOH (100 g NaOH in 1 L water). Mix, and discard the solution.
When EtBr conc. is >0.5 µg/ml:
1.Add 29 g Amberlite XAD-16 (Rohm and Hass) for 1 L of solution.
2.Store the solution for 12 hr at room temperature, shaking it intermittently.
3.Filter the solution through a Whatman No. 1 filter, and discard the filtrate.
4.Seal the filter and Amberlite resin in a plastic bag, and dispose of the bag in the hazardous waste.
