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文献和实验Introduction Ethidium bromide (EtBr), 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and other methods
(210 ml HCl in 1 L water) . Mix and allow the solution to stand for several hr at room temperature. 3.Add 1 volume of 2.5 N NaOH (100 g NaOH in 1 L water). Mix, and discard the solution. When EtBr conc. is >0.5 µg/ml: * electrophoresis buffer
Ethidium Bromide Decontamination
N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide. Decontamination of solutions > 0.5mg/ml This method reduces the mutagenic activity
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