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文献和实验Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Syringe (1-mL) (Fisher Scientific) Thermo plate (MATS) METHOD Experiment Set-Up 1. Prepare culture media (two dishes of cleavage medium with 10% SPS, and one dish of 2 mM DMAP): i. Place 10-15 drops
A Comprehensive Guide to Sleeping Beauty–Based Somatic Transposon Mutagenesis in the Mouse
70% ethanol TE buffer (see recipe ) 1.1× ReddyMix (Thermo Scientific
, without CaCl2 , MgCl2 , MgSO4 , 500ml bottle, Store at 4° C. Glacial Acetic Acid: FISHER SCIENTIFIC cat# A38-500. Acetic acid, Glacial ACS (Aldehyde free), 500ml bottle. L-Glutamine: GIBCO cat# 25030-032. L-Glutamine solution 100X (200mM), 20ml bottle
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