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文献和实验. Incubate for 15 min at 37 °C. Rinse each flask x 2 with 10 ml DPBS (without Ca or Mg). 6) Add 4 ml of Trypsin to each flask. Incubate at 37 °C for 3 min. Neutralize with 8 ml of Trypsin Neutralization Solution. 7) Wash
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Syringe (1-mL) (Fisher Scientific) Thermo plate (MATS) METHOD Experiment Set-Up 1. Prepare culture media (two dishes of cleavage medium with 10% SPS, and one dish of 2 mM DMAP): i. Place 10-15 drops
50‐ml conical polypropylene centrifuge tubes (Corning and Thermo Scientific) Refrigerated centrifuge (Sorvall RC‐5B or equivalent
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