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文献和实验Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Syringe (1-mL) (Fisher Scientific) Thermo plate (MATS) METHOD Experiment Set-Up 1. Prepare culture media (two dishes of cleavage medium with 10% SPS, and one dish of 2 mM DMAP): i. Place 10-15 drops
384‐well optical tissue culture plates, sterile (Greiner Bio‐One, cat. no. EK‐30091) 16‐channel electronic pipettor, 2.0‐125 µl (Thermo Scientific, cat. no. 2061
Site‐Specific Protein Labeling with SNAP‐Tags
Cell‐impermeable BG‐S‐S‐Alexa Fluor 488 (for probe synthesis, see Cole and Donaldson, ) TCEP (Sigma or Thermo Scientific; a 500 mM stock is prepared in water
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