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文献和实验between rounds of immunodetection. Any residual antibody molecules will bind permanently to the membrane if it is allowed to dry. back to top Re-Blot Plus Western Blot Recycling Kit The Re-Blot Plus Western Blot Recycling Kit contains
min at room temp. Wash 3 x 10 min with 0.05% Tween 20 in PBS. Detect with Amersham ECL kit (RPN 2106). I. Stripping blot Rinse blot off with 0.05% Tween 20 in PBS. Put blot into Kapak bag cut to slightly bigger
-Western blotting technique is quite similar to Western blotting. In a Western blot, an antibody is used to detect the corresponding antigen on a membrane. In a classical far-Western analysis, a labeled or antibody-detectable “bait” protein is used to probe
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