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- 2025年09月07日
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- 文献和实验
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4 x 1.25 mL
描述
Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.10X Taq Buffer with KCl and 15 mM MgCl2 (B16) includes:
• 100 mM Tris-HCl (pH 8.8 at 25°C)
• 500 mM KCl
• 0.8% (v/v) Nonidet P40
• 15 mM MgCl2
Related Products
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Taq Buffer with (NH4)2SO4 (10X)
内容及储存
• 4 x 1.25 mL 10X Taq Buffer with KCl and 15 mM MgCl2
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文献和实验of a master mix containing water, buffer, dNTPs, primers and Taq DNA Polymerase in a single tube, which can then be aliquoted into individual tubes. MgCl2 and template DNA solutions are then added. This method of setting reactions minimizes the possibility
mM Tris 7.9 5 ml Tris pH 7.950 mM KCl1.86 g KCl1 mM EDTA 1 ml 0.5M EDTA0.5% Tween 20 5 ml 50% Tween 200.5% NP-40 5 ml 50% NP-40up to 500 ml with QStore at room temperature and add PMSF to a final concentration of 1 mM just prior to use.Buffer C50 mM
: 6.61 g Tris-HCl + 0.8 g Tris-Base (pH 7.4 at 25℃)C: 1 M MgCl2 10 ml: 2.03 gD: 3 M NaCl 20 ml: 3.50 gE: 2M KCl 10 ml: 1.48 gBan I: 0.3ml B + 0.3ml C + 0.10ml D + 0.1ml BSA + 21µl bME + 4.18ml H2OBgl II: 0.5ml B + 0.5ml C + 1.67ml D + 0.1ml BSA + 35µl bME
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