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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
phi29 DNA Polymerase (10 U/µL)
- 规格：
1,000 units
描述
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA. Therefore 3'-modified primers are highly recommended.Highlights
• Highest processivity and strand displacement activity among known DNA polymerases – more than 70 kb long DNA stretches can be synthesized
• Highly accurate DNA synthesis
• Extremely high yields of amplified DNA even from minute amounts of template
• Amplification products can be directly used in downstream applications (PCR, restriction digestion, SNP genotyping, etc.)
规格
| Polymerase: | phi29 DNA Polymerase |
|---|---|
| Product Size: | 1,000 units |
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文献和实验otherwise. Multiply primed rolling circle amplification (MPRCA) and multiple displacement amplification (MDA) methods are based on the same principle. The DNA amplification is non-PCR based and uses Φ29 DNA polymerase and random hexamer primers for unbiased
Advantages Cat. No. Taq DNA Polymerase •Sequencing•Safeguarding sample•In vitro transcription TOPO® Cloning(5 minutes) Topoisomerase TOPO TA Cloning® Kit •≥95
PCR(Polymerase Chain Reaction) 即聚合酶链式反应，对于常年狂奔在实验室的实验狗来说并不陌生。那么，对于 PCR 反应的核心成份，DNA 聚合酶，你选对了吗？ 常见的 DNA 聚合酶如 Taq、Phanta、Pfu、KOD、Primerstar、Klenow、Bst、Phi29 等等。根据耐热性来分可分为耐热酶和常温酶。耐热聚合酶如 Taq、Pfu、KOD、Primerstar 等，常温聚合酶包括 Klenow、Bst、Phi29 等。根据保真度来分，高保真聚合
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