- ¥15348
- BD
- 558569
- 2025年08月22日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Human Naive CD8 T Cell Enrichment Set - DM
- 规格：
558569
Description
The BD IMag™ Human Naïve CD8+ T Cell Enrichment Set - DM is used for the negative selection of untouched naïve CD8+ T cells from peripheral blood. The Human Naïve CD8+ T Cell Enrichment Cocktail contains biotinylated monoclonal antibodies that recognize antigens expressed on memory T cells, CD4+ T cells, B cells, NK cells, γ/δ T cells, monocytes, granulocytes, platelets, and erythroid cells. The BD IMag™ Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. With these two components, the BD IMag™ Human Naïve CD8+ T Cell Enrichment Set - DM avoids the inadvertent activation of the enriched naïve CD8+ T cells by using reagents that do not directly bind to those T cells. This Enrichment Set has been optimized for use with the BD IMag™ Cell Separation Magnet, and it contains sufficient reagents to label 10e9 peripheral blood mononuclear cells (PBMC).
The Human Naïve CD8+ T Cell Enrichment Cocktail, 5.0 mL, is comprised of the following monoclonal antibodies:
Biotin Mouse Anti-human CD4, clone L200
Biotin Mouse Anti-human CD11b, clone ICRF44
Biotin Mouse Anti-human CD16, clone 3G8
Biotin Mouse Anti-human CD19, clone HIB19
Biotin Mouse Anti-human CD41a, clone HIP8
Biotin Mouse Anti-human CD45RO, clone UCHL1
Biotin Mouse Anti-human γδ-TCR, clone B1
Biotin Mouse Anti-human Glycophorin A, clone GA-R2
Components
| Part No. | Description | Size |
|---|---|---|
| 51-9003746 | Streptavidin Particles Plus – DM | 7.5 mL (1 ea) |
| 51-9004781 | Human Naive CD8 T Cell Enrichment Cocktail | NA |
Suggested Companion Products
| Cat No. | Description | Size | |
|---|---|---|---|
| 555347 | PE Mouse Anti-Human CD4 RPA-T4 RUO | 100 Tests |
|
| 555466 | FITC Mouse Anti-Human CD41a HIP8 RUO | 100 Tests |
|
| 555388 | PE Mouse Anti-Human CD11b/Mac-1 ICRF44 (also known as 44) RUO | 100 Tests |
|
| 552311 | Cell Separation Magnet RUO |
|
|
| 552362 | Buffer (10X) RUO | 100 mL |
|
| 555407 | PE Mouse Anti-Human CD16 3G8 RUO | 100 Tests |
|
| 555413 | PE Mouse Anti-Human CD19 HIB19 RUO | 100 Tests |
|
| 555455 | PE Mouse Anti-Human CD36 CB38 (also known as NL07) RUO | 100 Tests |
|
| 555516 | PE Mouse Anti-Human CD56 B159 RUO | 100 Tests |
|
| 555493 | PE Mouse Anti-Human CD45RO UCHL1 RUO | 100 Tests |
|
| 555644 | PE Mouse Anti-Human CD123 9F5 RUO | 0.2 mg |
|
| 555570 | PE Mouse Anti-Human CD235a GA-R2 (HIR2) RUO | 0.1 mg |
|
| 555717 | PE Mouse Anti-Human TCR γδ B1 RUO | 0.1 mg |
|
| 550855 | APC Mouse Anti-Human CD45RA HI100 RUO | 100 Tests |
|
| 555349 | APC Mouse Anti-Human CD4 RPA-T4 RUO | 100 Tests |
|
| 559925 | 7-AAD RUO | 2 mL |
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文献和实验The generation of an antigen-specific T-lymphocyte response is a complex multi-step process. Upon T-cell receptor-mediated recognition of antigen presented by activated dendritic cells, naive T-lymphocytes enter a program of proliferation
Preclinical Testing of Strategies for Therapeutic Targeting of Human T-Cell Trafficking In Vivo
Naive T cells are migratory cells that continuously recirculate between blood and lymphoid tissues. Antigen-specific stimulation of T cells within the lymph nodes reprograms the trafficking properties of T cells by inducing a specific set
T-cell dependent antigen specific in situ staining (Pe)
). Double staining (TUNEL): 19- warm the section in TBS 20- prepare a warm incubation box 21- set up the TUNEL mixture (see data sheet) 22- incubate 30 min at 37°C. 23- wash thrice in TBS-T 24- apply goat anti-FITC-HRP 1:200 25- wash twice
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