- ¥4727
- bd
- 558564
- 2025年08月22日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Alexa Fluor® 647 Mouse Anti-Human NKp44 (CD336
- 规格：
100t
BD Pharmingen
Alternative Name
CD336; NCR2; NCTR2; NKp44; NK-p44; LY95; dJ149M18.1
Vol. Per Test
20 µl
Isotype
Mouse BALB/c IgG1, κ
Reactivity
Human (QC Testing)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human NKp44
Entrez Gene ID
9436
Description
The p44-8 monoclonal antibody specifically binds to the natural killer (NK) cell receptor, NKp44, which is also known as CD336, Natural cytotoxicity triggering receptor 2 (NCR2), or Lymphocyte antigen 95 homolog (Ly95). NKp44 is a ~44 kDa type I transmembrane protein that belongs to the natural cytotoxicity receptor (NCR) family within the immunoglobulin superfamily. NKp44 is expressed by activated NK cells. NKp44 serves as an activating receptor that can enhance NK cell mediated lysis of target cells including tumor cells and virus-infected cells. Killer activating receptor associated protein (KARAP), which is also known as DAP12, is an intracellular adaptor protein that can associate with the intracellular region of NKp44. DAP12 can then function to help transmit activating signals through its immunoreceptor tyrosine-based activation motif (ITAM).
Format
- Format
- Alexa Fluor® 647
- Excitation Source
- Red 633 nm
- Excitation Max
- 650 nm
- Emission Max
- 668 nm
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
体，实现了对抗原特异性 (CD8+ T) 细胞的检测和分离 (如，流式分析) 。此技术的特点在于，荧光或磁性微珠等标记物可以通过使用 Biotin (生物素) 而去除，保留了细胞的真实特性，得到的功能性的，label-free 的细胞可用于下游的实验研究。 1) MHC I STREPTAMERS® 技术解析 MHC I STREPTAMERS® 由两部分组成： · MHC I-Strep，即连接了 Twin-Strep-tag® 和抗原肽的 MHC I 分子。 · 荧光 (PE/APC)
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
技术资料暂无技术资料 索取技术资料
