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- ZYMO
- R1200-25
- 2025年09月09日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
DNA/RNA Shield(2X Concentrate)
- 规格：
25ml
ZYMO DNA/RNA Shield
(2X Concentrate)
，DNA/RNA保存液
(2X Concentrate)
| R1100-50 | DNA/RNA Shield | 50 ml |
| R1100-250 | DNA/RNA Shield | 250 ml |
| R1200-25 | DNA/RNA Shield (2X Concentrate) | 25 ml |
| R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml |
ZYMO DNA/RNA Shield-DNA/RNA保存试剂是用于任何生物样品中核酸的DNA和RNA稳定溶液。这种DNA和RNA稳定溶液在环境温度下保持了样本的遗传完整性和表达谱，并完全灭活了传染源（病毒、细菌、真菌和寄生虫）。独特的DNA和RNA稳定溶液还可以防止冻融循环和意外的冷冻失败导致的降解。
| Applicable For | Next-Generation Sequencing, qPCR, microarray. |
|---|---|
| Device Specs | Reagent |
| Reagent Storage | Ambient temperature => 2 years |
| Sample Collection | 10% (v/v or w/v) |
| Sample Source | Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc. |
| Sample Stability | RNA: Ambient temperature (4°C- 25°C) >1 month DNA: Ambient temperature (4°C- 25°C) >2 years DNA & RNA: Frozen (<-20°C): Indefinitely |
问题1：解冻保存在DNA/RNA保存液中的冷冻样品后出现白色沉淀，这是正常的吗？
在冻融过程中，试剂（或样品）的某些成分可能从溶液中沉淀出来。一旦样品解冻到环境温度，旋涡样品，使沉淀物回到溶液中。此外，试着用手或37°C加热样品5分钟，然后涡流。
问题2：在储存之前，我是否需要在Shield priorDNA/RNA 中对样品进行均质处理？
不，组织不需要均匀化。
问题3：如何在DNA/RNAShield中收集固体组织？
在室温下将组织转移到DNA-RNAShield层，浸泡。在组织匀浆之前，可以将组织匀浆或冷冻保存。
Q4: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 ul of FACS sorted cells into minimally 400 ul of DNA/RNA Shield).
Q5: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°C): Indefinitely
Q6: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month DNA > 2 years
Q7: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q8: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
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文献和实验RNA in situ protocol for DNA and RNA probes
) to detect the hybridizing RNA in whole embryos. The major modification made was to increase the number of post-hybridization washes in hybridization buffer (50% deionized formamide, 5x SSC, 200 ug/ml tRNA, 100 ug/ml sonicated, boiled salmon sperm DNA
Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation
m g of Cot1 human DNA (20 m g/ m l, Gibco-BRL), 20 m g polyA RNA (10 m g/ m l, Sigma, #P9403) and 20 m g tRNA (10 m g/ m l, Gibco-BRL, #15401-011). Spin 7-10 min. at 14,000 x g. Look for concentration of the probe in the microcon. The probe usually
RNA in situ protocol for DNA and RNA probes
RNA in situ protocol for DNA and RNA probes This procedure was adapted from one originally described by Tautz and Pfeifle (1989). It makes use of digoxigenin labelled probes and alkaline phosphatase-conjugated anti-digoxigenin
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