
细胞质和核RNA纯化试剂盒(提取试剂盒) 现货|Cytopl
asmic & Nuclear RNA Purification Kit (50)研选同类产品更多 >
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- 保存条件:
溶液密封室温保存,未开封至少保存1年。
- 保质期:
1年
- 英文名:
Cytoplasmic & Nuclear RNA Purification Kit (50)
- 库存:
100
- 供应商:
艾美捷科技
- 规格:
50Preps
产品名称:细胞质和核RNA纯化试剂盒(提取试剂盒) 现货-Cytoplasmic & Nuclear RNA Purification Kit (50)
产品货号:NGB-21000
产品规格:50Preps
背景资料:
在某些情况下,希望能够分离分馏的RNA而不是总RNA。例如,对于表达谱分析的一些研究,可能优选仅分离成熟的细胞质RNA。或者,可能需要分离核RNA以研究和研究预处理的(非剪接的)RNA。 此外,该试剂盒可用于分离RNA,用于需要避免DNA污染的下游应用,因为已显示细胞质部分不含任何痕量的基因组DNA。
保存建议:
细胞质和核RNA纯化试剂盒(提取试剂盒) 现货-Cytoplasmic & Nuclear RNA Purification Kit (50)溶液密封室温保存,未开封至少保存1年。
产品描述:
此试剂盒提供了从培养的动物细胞和小组织样品中分离和纯化细胞质和核RNA的快速方法。该试剂盒可用于从细胞质和核RNA组分中分离所有大小的RNA,包括所有小RNA种类,无需任何苯酚。试剂盒中包括足够的试剂,可以进行50次细胞质RNA制备或25次细胞质和25次核RNA制备。可在约45分钟内处理十个样品。
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文献和实验Chen X, Long noncoding RNA MHENCR promotes melanoma progression via regulating miR-425/489-mediated PI3K-Akt pathway,American Journal of Translational Research. 2017.
Codrich M,Neuronal hemoglobin affects dopaminergic cells’ response to stress,Cell Death and Disease. 2017.
Kishikawa T, Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1,Nature Communications. 2016.
Kouwaki T, Extracellular Vesicles Including Exosomes Regulate Innate Immune Responses to Hepatitis B Virus Infection,Frontiers in Immunology. 2016.
Reisman, D.,Evidence for a role of a lncRNA encoded from the p53 tumor suppressor gene in maintaining the undifferentiated state of human myeloid leukemias,Gene Reports. 2016.
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
5 seconds again. Quick spin 30 seconds at maxi speed at RT. 6: Transfer cytoplasmic fraction to a new tube, put nuclear samples on ice now. 7: T℃ytoplasmic fractions, add 0.9ml 1.2xTRIzol reagent. Vortex to mix completely, sit at RT for 5 minutes
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. Vortex the tube for 30 seconds. 18. Centrifuge at maximum speed ($13000 x g) at 4°C for 3 minutes. Aspirate the supernatant and air dry the RNA pellet by inverting the tube on a absorbent paper for 5-10 minutes 19. Add 50-100μl of DEPC












