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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human epidermal keratin.
- 形态:
PBS, pH 7.4Label: FITCState: PurifiedState: Liquid purified IgG fractionStabilizer: 1% BSAPreservative: 0.05% Sodium Azide
- 保存条件:
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM10028FC-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
FC, IF
- 抗体英文名:
pan Cytokeratin mouse monoclonal antibody, clone AE1/AE3, FITC, Purified
- 抗体名:
pan Cytokeratin mouse monoclonal antibody, clone AE1/AE3, FITC, Purified
- 规格:
200 ug
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文献和实验)- A mixture of two anti-cytokeratin monoclonal antibodies AE1 and AE3.- Source: Biogenex.- Dilution 1: 400.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, typically fibrillary, with or without accentuation
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Direct Immunofluorescence Labeling
Recipes Fluorochrome-Conjugated Antibodies: Test antibody: Mouse monoclonal conjugated to FITC, PE, PerCP or PE-CY5 Tandem (tradenames: Cy-Chrome or Tri-Color). If you''re planning to label cells with 2 or more antibodies simultaneously
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