- 询价
- R&D
- ZK-0002453
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Pan-Cytokeratin (AE1/AE3) Monoclonal Antibody, Purified (SIGNET)详细信息：
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Description Purified Monoclonal Antibody against Pan-Cytokeratin (AE-1/AE-3)
(Formerly Signet Catalog Nos: 464-01, 464-16 L1 Predilute, 464-26 L2 Predilute, 464-36 LV Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Clone AE-1/AE-3 Form Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse Species Reactivity Human IsoType Pan-Cytokeratin (AE1/AE3) Monoclonal Antibody, Purified (SIGNET)IgG1 Specificity The pan-cytokeratin AE-1/AE-3 antibody cocktail provides a broad spectrum of reactivity and stains low molecular weight and high molecular weight cytokeratins in virtually all epithelia.
This antibody was developed using human epidermal keratins.
Concentrated Format:
SIG-3464-1000 [1 mL]
Prediluted Formats:
SIG-3464-16 [6 mL] is ready to use with Peroxidase Anti-Peroxidase detection systems.
SIG-3464-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
Pan-Cytokeratin (AE1/AE3) Monoclonal Antibody, Purified (SIGNET)SIG-3464-36 [6 mL] is ready-to-use with Biotin based detection systems on Ventana ES and Ventana NexES automated immunostainers.Uses This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250)
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All™ 1 (SIG-31910).
Incubation: 20 minutes at room temperature
Notes Positive tissue (human): Skin - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250)
- Pan-Cytokeratin (AE1/AE3) Monoclonal Antibody, Purified (SIGNET)
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文献和实验Monoclonal Antibody Affinity Chromatography
all the above criteria and also afford the advantage of being available in essentially unlimited supply. Moreover, they are themselves readily purified, often by affinity chromatography. The main disadvantage of monoclonal antibody ligands is that they must possess
Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography
proteins. Taking advantage of the exquisite specificity afforded by each antibody results in a final purified product that binds specifically to that MAb. Using immunoaffinity chromatography, a single antigen can be separated from very complex mixtures (e.g
)- A mixture of two anti-cytokeratin monoclonal antibodies AE1 and AE3.- Source: Biogenex.- Dilution 1: 400.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, typically fibrillary, with or without accentuation
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