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- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Mouse tropomyosin 2, beta (cDNA clone MGC:18587 IMAGE:3497670), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
- 供应商:
OriGene
- 规格:
20 ug
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Figure 27.4.1 Schematic diagram of overall protocol. Yeast clones containing cDNA constructs are plated on selection medium. Each clone is grown and protein expression is induced. Pellets of each clone are transferred into 96‐well boxes. Fusion
cDNA Expression Cloning in Mammalian Cells
) Recombinant T7 (Fuerst et al., ) or SP6 (Usdin et al., ) vaccinia virus stock (available from B. Moss, NIH) Purified DNA from cDNA pools (see protocol 1 , steps
) If the particular vector, or phage, used to construct a cDNA library contains a promoter region upstream of the insertion site we may be able to screen for desired clones by looking for expression of the protein of interest .
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