pGpx4-1-Fluc plasmid

pGpx4-1-Fluc plasmid

PVT111534

pGpx4-1-Fluc plasmid Information

Plasmid name: pGpx4(mouse)-1-Fluc
Alias:
Gene lenght: 744
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: Gpx4
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pGpx4-1-Fluc plasmid Description

pGpx4-1-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pGpx4-1-Fluc plasmid Sequence

1 ggtaccCAAG ATAGGGCCTG GTGTCATTGA CCTATCTATC ACCCTAGTAC CTAGGAGTCT 61 AAGGCAGGAT TGCTGAGTTC TAAGACCGGC TGAACTGTAA AATTGGGGGT CGAGGGGGGG 121 GGGAGAAGCT ACATCTGGGG TTCGGGACTC AATGGGTTGA GAGCCCTCAC TTGCATGCTG 181 CAGGTTCTGG GTTCCATCCC TACACCTCAT GGCAGCACAC TTTTGCCATT CCATAGTTTA 241 GGTTAGCCTG AGCTACAGGA GGCAACCTCT GATAAAGAAA ATAGAAATGG AAGGGATGTG 301 GGGATTGGCC GACACCGAGA TCCCAGCACT CAGTAGGCTG AGGCTGGCGG TCCAGGGGTT 361 CAAGGTCATC CTCTGCTATA TAAACAGTCC AAGGCTAGCC TGGGTTACAT GAGATTCTGT 421 TTCCAGTAGA AAAAGGAGAC TCCTGTACTT TCAAAGGGGC TCTTTAAGGG GATGACTTTG 481 ACACGCTTTC TATTCCCAGA GGTTCCCCAA AGCGCCCTGG CACCCTGCTC CGCTGCAGGC 541 AACCTCGCGG ATAACTGTCC ACAGCCCTCC AACGCGTCGT CCGCGAGAAC GCGGCTCCCG 601 TTTCTCCCGC GTCCCTATCA CTGGGCATGC GCAGTTGCTG ATAACAACAA ACTCGTAAGC 661 CCAGCCCCGC CCAAGCCGTC CCTTCATTCA GGCTTCCCAT TGGCTGCAGG GGCCTCGCGT 721 CTTAGCGCAG GCGCATCTGA CCAATAAGAG aagcttggca ttccggtact gttggtaaag 781 ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag 841 atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa 901 caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa 961 tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg 1021 tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg 1081 gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg 1141 gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg 1201 tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc 1261 agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat 1321 acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct 1381 ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat 1441 tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa 1501 gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg 1561 gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg 1621 attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca 1681 ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc 1741 tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag 1801 gatatgggct cactgagact acatcagcta ttctgattac acccgagggg gatgataaac 1861 cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg 1921 ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt 1981 ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac 2041 attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga 2101 agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc 2161 tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg 2221 aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg 2281 tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg 2341 tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc 2401 tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc 2461 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc 2521 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta 2581 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg 2641 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata 2701 aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg 2761 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga 2821 caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 2881 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 2941 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3001 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3061 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3181 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 3241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 3361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 3421 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 3481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 3541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 3601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 3661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 3721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 3781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 3841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 3901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 3961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 4021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 4081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 4141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 4201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 4261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 4321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 4381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 4441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 4501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 4561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 4621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 4681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 4741 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc 4801 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 4861 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 4921 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 4981 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 5041 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 5101 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 5161 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac 5221 aatttcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 5281 ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt 5341 acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg 5401 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta 5461 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.