pLV3-CMV-RAMP1-EF1a-mCherry-Pu

pLV3-CMV-RAMP1-EF1a-mCherry-Puro plasmid

PVT107607

pLV3-CMV-RAMP1-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-RAMP1(human)-EF1a-mCherry-Puro
Alias: NM_005855.4
Gene lenght: 444
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-RAMP1-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-RAMP1-EF1a-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-RAMP1-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggcccg ggccctgtgc cgcctcccgc ggcgcggcct 2341 ctggctgctc ctggcccatc acctcttcat gaccactgcc tgccaggagg ctaactacgg 2401 tgccctcctc cgggagctct gcctcaccca gttccaggta gacatggagg ccgtcgggga 2461 gacgctgtgg tgtgactggg gcaggaccat caggagctac agggagctgg ccgactgcac 2521 ctggcacatg gcggagaagc tgggctgctt ctggcccaat gcagaggtgg acaggttctt 2581 cctggcagtg catggccgct acttcaggag ctgccccatc tcaggcaggg ccgtgcggga 2641 cccgcccggc agcatcctct accccttcat cgtggtcccc atcacggtga ccctgctggt 2701 gacggcactg gtggtctggc agagcaagcg cactgagggc attgtgtagg gatccgcggc 2761 cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag 2821 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg 2881 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 2941 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca 3001 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct 3061 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct 3121 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg 3181 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg 3241 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac 3301 cggcgcctac gctagacgcc accatggtga gcaagggcga ggaggataac atggccatca 3361 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 3421 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 3481 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 3541 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 3601 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 3661 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 3721 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 3781 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 3841 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 3901 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 3961 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 4021 agctgtacaa gggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg 4081 agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 4141 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 4201 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 4261 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 4321 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 4381 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 4441 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 4501 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 4561 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 4621 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 4681 agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 4741 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 4801 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 4861 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 4921 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 4981 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 5041 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 5101 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 5161 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 5221 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc 5281 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 5341 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg 5401 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 5461 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 5521 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 5581 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 5641 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 5701 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 5761 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 5821 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 5881 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga 5941 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg 6001 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6061 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 6121 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 6181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 6241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 6301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 6361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 6421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 6481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 6541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 6601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 6661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 6721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 6781 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 6841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 6901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 6961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 7621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 7681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 7741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 7801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 7861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 7921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 7981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8101 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 8161 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 8221 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 8281 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 8341 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 8401 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 8461 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 8521 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 8581 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.