pPLIN3-Fluc plasmid

pPLIN3-Fluc plasmid

PVT111522

pPLIN3-Fluc plasmid Information

Plasmid name: pPLIN3(human)-Fluc
Alias:
Gene lenght: 2000
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: PLIN3
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3（special construction）
Reverse primer:
Copy number: High
Induction:

pPLIN3-Fluc plasmid Description

pPLIN3-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pPLIN3-Fluc plasmid Sequence

1 ggtaccggac tcaataaata tggttgaatg agtgaatgag aaggaaaaag cctgttacaa 61 atgtcatgga atggggtctc agattcactt gagaatctca ctagctgcct gaagttactt 121 aaatttaaac attcaggcca agcgcggtag ctcacacctg taatcccagc actttaggag 181 gccgaggcag gtggatcact tgaggccagg agttcgagac cagcctggcc aatatggtta 241 gtcctcgtct ctactaaaaa tacaaaaatt agctgggtgt ggtggcgcgc gcctgtaatc 301 ccagctactt gggaggctga ggcaggagaa ccgcttgaac ccgggaggtg gaggttgtag 361 tgagtcgaga tcgcgccatt gcactccagc ctgggagaca gagcgagact ctgcctcaaa 421 aaaaaaaaaa aaatttaaac attcagcttc tcactgtgca ttagccacgt ggtgcttttg 481 gctacaatgt tggatggcac acaattctag aattgcagaa agttctattg gacagtgcta 541 gcatagaccc tgtccccact caccccccca aaaaaatgca gtcacagaca cccaattttt 601 cccatgttct ttctaaagct accagaagct tcattctatt caaataattt ggcagaggtg 661 gcaaactggt ggcccctggg ctggacaaag ccccagttgg gtttcatttg gtccaattgt 721 tccctaacag gcaggacatg tctacagctt gaacttcctg tggatctgtg actctccagg 781 tgggtctccc atagggcact gctctcccct gcccctctaa gcagggcatc actgccacct 841 cctcagcaga aatattcacc tcctccctgg accatctgag attgagacct tggtttatag 901 accaagagga taaataattt gggaggtgga gaggggcggg gtaaaatcca agtgggggca 961 gcttcaggaa aaggttgatc cagcagtcac agagcctgga gcctgacccc tccattgccc 1021 ctggctgacc ctctccaggc ctcagtttcc ttctctgcaa aatagggatg attaaaaaga 1081 caatacagtg ctgggaggat gaagtggtct gatgttgggc agatcaggcc ggaggctgga 1141 caagttcccg tggtggttca agtcactcat atagctgggt tgggccgggt gcggtggctc 1201 atacctgtga tcccagcaat ttgggaggcc caagcgggca gatcacttga ggtcaggagt 1261 tcaagaccaa catggtgaaa ccccgtctct gctaaaaata caaaaaatta gccgggcgtg 1321 gtggcacgtg tctgtaatcc cagctactca ggaggctgag gcaggagaat cgcttgaatc 1381 caggaggtgg aggctgcagt gagccaaggt cgcgtcactg cactccagcc ttagcgacag 1441 agcgagactg tgtctcaaaa aaaaaaaaaa agtgtgtgtg tgtgtgtgtg tgtgtgtgtg 1501 tacatatagc tgagtcggag cccatggtgg agggcgtggc ccctgacgcg ctggcgaggg 1561 tcaccctgca actctcccag ggttctcgca gcctcagttt ggccataggg gaaatgggcg 1621 acctggggaa tcccagcacc tgtgcggcgc ccccgatcgc actcacctcc cagggccaac 1681 ttcacagggt ccccagctgc ggagcggggg tcccgtggct gggacaggcg tggtccaaga 1741 gtgctccctc cccttcaggg gcagggcgtg gagtccttcc cggacttcat gacttggcga 1801 ggggcggggc ctagtggagg cggggcaggg aggagagggg cgggcctggc gcgggcaatc 1861 cctcaacctg attggtcccc tcgcccgtca ctccagtgcg cccccaacct accacgcagt 1921 aaaagccacc cccgcctcgg cccggacggt ttccaagctg gttttgaagt cgcggcagct 1981 gttcctggga cgtccggttg accgcgaagc ttggcattcc ggtactgttg gtaaagccac 2041 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 2101 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 2161 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 2221 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 2281 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 2341 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 2401 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 2461 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 2521 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 2581 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 2641 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 2701 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 2761 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 2821 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 2881 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 2941 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 3001 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 3061 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 3121 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 3181 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 3241 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 3301 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 3361 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 3421 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 3481 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 3541 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 3601 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 3661 aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg gccggccgct 3721 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 3781 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 3841 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 3901 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga 3961 tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 4021 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg 4081 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4441 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 4501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 4741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 4861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 4921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 4981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 5821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 5881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 5941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6001 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 6061 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 6121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 6181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 6241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 6301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 6361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 6421 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 6481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 6541 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt 6601 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg 6661 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa 6721 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.