pLV3-CMV-Hmga1-EF1a-CopGFP-Pur

pLV3-CMV-Hmga1-EF1a-CopGFP-Puro plasmid

PVT107165

pLV3-CMV-Hmga1-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Hmga1(mouse)-EF1a-CopGFP-Puro
Alias: NM_001025427.3;Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy
Gene lenght: 324
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Hmga1-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-Hmga1-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-Hmga1-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga gcgagtcggg ctcaaagtcc 2341 agccagcccc tggcctccaa gcaggaaaag gatgggactg agaagcgagg ccggggcagg 2401 ccacgcaagc agcctccggt gagtcctggg acggcgctgg tcgggagtca gaaagagccc 2461 agtgaagtgc caactccgaa gagacctcgg ggccgaccaa agggaagcaa gaataagggc 2521 gccgccaaga cccggaaagt caccacagct ccagggagga aaccaagggg cagacccaag 2581 aaactggaga aggaggaaga ggagggcatc tcccaggagt cctctgagga ggagcagtga 2641 ggatccCTGC GATATCCAGC gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 2701 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 2761 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 2821 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 2881 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 2941 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3001 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3061 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 3121 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 3181 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 3241 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 3301 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 3361 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 3421 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 3481 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 3541 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 3601 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 3661 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 3721 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 3781 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 3841 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 3901 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 3961 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4021 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4081 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4141 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4201 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 4261 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 4321 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 4381 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 4441 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 4501 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 4561 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 4621 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 4681 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 4741 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 4801 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 4861 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 4921 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 4981 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 5041 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5101 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5161 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct 5221 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 5281 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 5341 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 5401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 5461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 5521 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 5581 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 5641 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 5701 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 5761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 5821 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 5881 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 5941 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 6001 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 6061 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 6121 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 6181 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 6241 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 6301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6721 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 6781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 7021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 7081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 7141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 7201 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 7261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7621 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 8041 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 8101 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 8161 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 8221 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 8281 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 8341 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 8401 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 8461 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 8521 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.