pALDOA-Fluc plasmid

pALDOA-Fluc plasmid

PVT111276

pALDOA-Fluc plasmid Information

Plasmid name: pALDOA(human)-Fluc
Alias: NG_008010.1
Gene lenght: 2000
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: ALDOA
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pALDOA-Fluc plasmid Description

pALDOA-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pALDOA-Fluc plasmid Sequence

1 ggtaccATCC CAGCACTTTG GGAGACCAAG GTGGGTTTAG CCCAGGAGTT TGAGACTAGC 61 CTGGGCAACA TGATGAAACC CTGTCTCTAC AAAAAAATAC AAAAAATGAA TTAGCCAGGC 121 GTGGTGGCAC GTGCCTGTAA TCCCAGCTAC TTGGGAGGCT GAGGCAGGAG AATCGCTTGA 181 ACCCAGGAGG CGGAGGTTGC AGTGAGCCGA GACCGCGCCA TGGCACTCCA GCCTGGCAAC 241 AGAGCGAGAC TCCGTCTCAA AAAAAAAAAA AAAAAAGTAC AAAAAATGAG CCGAGCACGG 301 TGGTTCATGC CTGTAATCCC AGCACTTTGG GAGGCCAAGG CGGGCAGATA CCTGAGGTCA 361 GGAATTCAAG ACCAGCCTGG TCAACATAGC GAAACCCTGT CTCTACTAAA AATACAAAAT 421 TAGCCAAGTG TGGTGGCACA TGCCTGTAAT CCCACCTACT TGGGAGGCTG AGGCAGGAGA 481 ACCGCTTGAA TCCAGAGGTG GAAGTTGCAG TGAGTGGAGA TCACGCCATT GTGCTCCAGC 541 CTGGGCAACA GGAGTGAAAC TCCATCTCGG AAAAAAAAAA AAAAAAATGG CCGGGCACAG 601 TTGCTCACTC CTTTAATCTT AGCACTTTGG GAGGCTGAGG CAGGTGGATC ATCTGAGGCC 661 AGGAGTTCCG AGACCAGCCT GACCAACATG GTGAAACCCC CATCTCTACT ACAAATACAA 721 AAATTAGCTG GGTGTGGTGG CTCACGCCTG TAGTTCAGCT ACTCAGAAGG CTGAGGCAGA 781 ACTACTTGAA CCCAGGAGGT GGAGGTTGCA GTGAGCCAAG ATCGCACCAT TGCACTCCAC 841 CCTGGGCAAC AGAGTGAGAC CCTGTCTCAA AAAAAAAAAA AAAATTAAAA ATAGGTAAAT 901 TGCCATTTTC AAAGATACCT GTAAAGATTT TTAAAATCAT TCATGAATGC GGTCTGTTCG 961 TTGCACAGAG TAGATGCTCA AAAATGGTGA ATGAGACCCT CTATTTTGGT CTCATGCTGA 1021 AGAAGTCCAT AAAGCCCACA AGTCATTTTC ATGATGGACA GAAAAATGTG TGTGCTTTCT 1081 CTGTCTACTG CCTCAACTGC ACAGACCCCG GGACTGTAGC AGAACCATCT TTTGAGCTTG 1141 ACACCGGGAG GCCCAAATTC TAGCACGGGA CCCAGGGCCA GTTGCTCTCT GGTCCTCAGT 1201 CTCCTCACCC ATAAAATGGG AAGGAGAGAA CCCTGAATCA TTGCTTCTAG CTTCTGAACT 1261 CAGTTGTTCA GAACAAGGAC TCACTGCTGA TTTTTCAACA GCACAGGGAA TTGCACTGTT 1321 CCTGGGAATG ATGGACAGTA CCCTCTGTTC CACTGGGCAA GTGAGATTTC CCAGGCCTCT 1381 CTGTTCCCTT CTCCCCTTAG AGAGCAACAG ACGTGGCCCC ATCCCACCTC CCTCAACCCT 1441 CTCTCTCCTC CCTCAGGACT GGGCACCTCG CTGCCCCCGC TGCTGCCCAC TCTGCGACTG 1501 TGCCTGTACG TGCCAGCTCC CCGACTGCCA GAGCCTCAAC TGTCTCTGCT TCGAGATCAA 1561 GCTCCGATGA GGACCCAGGG CCCCTGCCCT CTGGGGAGCG GCCAGCCCCC AGGGCCCATG 1621 TGCCCTCCTC CCTGAAGAGC CTTTCCCCAC GCCACTGGAA CCACAGATGG CCTGCCGAGC 1681 ACCCAGGCCT GGGAACTGGA AGTGGCAGCG CAGGGCCTGG CTCCCTGCAG GGCAGGACTC 1741 TTGGCCGGCT GGACGGCAGC TCCTCTGGAG GGCCAGAAAA GAGAGGGGCT AGTGCTCGGG 1801 CAGGTGCCCT GGCTTCCCTT CCCCTCCACA CGTCAACGAT TCTATTTGAA GTTGGGCAGG 1861 GGGGTGGCGC TGCTCACCAC ACACAAGTGT TATAGGAGGA GTCTGGCCCT TGAGTACCGG 1921 GTACGCAGGG GTGCCTCAAC CACACTCCGT CCACGGACTC TCCGTTATTT TAGGAGGTGA 1981 GTGTAGTGCC AGTATCTACT CTCCTTaagc ttggcattcc ggtactgttg gtaaagccac 2041 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 2101 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 2161 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 2221 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 2281 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 2341 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 2401 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 2461 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 2521 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 2581 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 2641 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 2701 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 2761 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 2821 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 2881 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 2941 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 3001 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 3061 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 3121 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 3181 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 3241 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 3301 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 3361 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 3421 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 3481 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 3541 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 3601 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 3661 aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg gccggccgct 3721 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 3781 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 3841 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 3901 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga 3961 tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 4021 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg 4081 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4441 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 4501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 4741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 4861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 4921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 4981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 5821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 5881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 5941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6001 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 6061 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 6121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 6181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 6241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 6301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 6361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 6421 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 6481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 6541 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt 6601 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg 6661 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa 6721 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.