pCAGGS-SYK-EGFP plasmid

pCAGGS-SYK-EGFP plasmid

PVT111265

pCAGGS-SYK-EGFP plasmid Information

Plasmid name: pCAGGS-SYK(human)-EGFP
Alias: NM_003177.7;p72-Syk
Gene lenght: 1905
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCAGGS-SYK-EGFP plasmid Description

pCAGGS-SYK-EGFP It is a plasmid suitable for gene expression in mammalian cells.

pCAGGS-SYK-EGFP plasmid Sequence

1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc 541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt 601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg 661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc 721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg 841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt 901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc 961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg 1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg 1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc 1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc 1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg 1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc 1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg 1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc 1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg 1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt 1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt 1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag 1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccacc 1741 atggccagca gcggcatggc tgacagcgcc aaccacctgc ccttcttttt cggcaacatc 1801 acccgggagg aggcagaaga ttacctggtc caggggggca tgagtgatgg gctttatttg 1861 ctgcgccaga gccgcaacta cctgggtggc ttcgccctgt ccgtggccca cgggaggaag 1921 gcacaccact acaccatcga gcgggagctg aatggcacct acgccatcgc cggtggcagg 1981 acccatgcca gccccgccga cctctgccac taccactccc aggagtctga tggcctggtc 2041 tgcctcctca agaagccctt caaccggccc caaggggtgc agcccaagac tgggcccttt 2101 gaggatttga aggaaaacct catcagggaa tatgtgaagc agacatggaa cctgcagggt 2161 caggctctgg agcaggccat catcagtcag aagcctcagc tggagaagct gatcgctacc 2221 acagcccatg aaaaaatgcc ttggttccat ggaaaaatct ctcgggaaga atctgagcaa 2281 attgtcctga taggatcaaa gacaaatgga aagttcctga tccgagccag agacaacaac 2341 ggctcctacg ccctgtgcct gctgcacgaa gggaaggtgc tgcactatcg catcgacaaa 2401 gacaagacag ggaagctctc catccccgag ggaaagaagt tcgacacgct ctggcagcta 2461 gtcgagcatt attcttataa agcagatggt ttgttaagag ttcttactgt cccatgtcaa 2521 aaaatcggca cacagggaaa tgttaatttt ggaggccgtc cacaacttcc aggttcccat 2581 cctgcgactt ggtcagcggg tggaataatc tcaagaatca aatcatactc cttcccaaag 2641 cctggccaca gaaagtcctc ccctgcccaa gggaaccggc aagagagtac tgtgtcattc 2701 aatccgtatg agccagaact tgcaccctgg gctgcagaca aaggccccca gagagaagcc 2761 ctacccatgg acacagaggt gtacgagagc ccctacgcgg accccgagga gatcaggccc 2821 aaggaggttt acctggaccg aaagctgctg acgctggaag acaaagaact gggctctggt 2881 aattttggaa ctgtgaaaaa gggctactac caaatgaaaa aagttgtgaa aaccgtggct 2941 gtgaaaatac tgaaaaacga ggccaatgac cccgctctta aagatgagtt attagcagaa 3001 gcaaatgtca tgcagcagct ggacaacccg tacatcgtgc ggatgatcgg gatatgcgag 3061 gccgagtcct ggatgctggt tatggagatg gcagaacttg gtcccctcaa taagtatttg 3121 cagcagaaca gacatgtcaa ggataagaac atcatagaac tggttcatca ggtttccatg 3181 ggcatgaagt acttggagga gagcaatttt gtgcacagag atctggctgc aagaaatgtg 3241 ttgctagtta cccaacatta cgccaagatc agtgatttcg gactttccaa agcactgcgt 3301 gctgatgaaa actactacaa ggcccagacc catggaaagt ggcctgtcaa gtggtacgct 3361 ccggaatgca tcaactacta caagttctcc agcaaaagcg atgtctggag ctttggagtg 3421 ttgatgtggg aagcattctc ctatgggcag aagccatatc gagggatgaa aggaagtgaa 3481 gtcaccgcta tgttagagaa aggagagcgg atggggtgcc ctgcagggtg tccaagagag 3541 atgtacgatc tcatgaatct gtgctggaca tacgatgtgg aaaacaggcc cggattcgca 3601 gcagtggaac tgcggctgcg caattactac tatgacgtgg tgaacgagct cggcggtgga 3661 ggaagcggcg gaggtggatc tatggtgagc aagggcgagg agctgttcac cggggtggtg 3721 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 3781 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 3841 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 3901 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 3961 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 4021 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 4081 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 4141 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 4201 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 4261 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 4321 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 4381 atggacgagc tgtacaagta gctcgagcta gcagatcttt ttccctctgc caaaaattat 4441 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc 4501 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca 4561 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg 4621 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct 4681 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt 4741 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc 4801 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga 4861 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 4921 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 4981 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 5041 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc 5101 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 5161 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 5221 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat 5281 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 5341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 5401 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5461 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5521 tcagctcact caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga 5581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 5641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 5701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 5761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 5821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 5881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 5941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 6001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 6061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 6121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 6181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 6241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 6301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 6361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 6421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 6481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 6541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 6601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 6661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 6721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 6781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 6841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 6901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 6961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 7021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 7081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 7141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 7201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 7261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 7321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 7381 aagtgccacc tgg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.