pLV3-CAG-Arhgap18-3×FLAG-Blast

pLV3-CAG-Arhgap18-3×FLAG-Blast plasmid

PVT107592

pLV3-CAG-Arhgap18-3×FLAG-Blast plasmid Information

Plasmid name: pLV3-CAG-Arhgap18(mouse)-3×FLAG-Blast
Alias: NM_176837.2;4833419J07Rik
Gene lenght: 1989
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pLV3-CAG-Arhgap18-3×FLAG-Blast plasmid Description

pLV3-CAG-Arhgap18-3×FLAG-Blast It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CAG-Arhgap18-3×FLAG-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATaagc ttgggagttc cggacattga ttattgacta gttattaata gtaatcaatt 1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2281 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg 2341 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt 2401 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg 2461 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag 2521 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 2581 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc 2641 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 2701 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt 2761 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg 2821 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg 2881 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg 2941 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac 3001 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc 3061 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt 3121 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag 3181 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg 3241 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 3301 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 3361 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 3421 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 3481 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 3541 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 3601 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 3661 tggcaaaGAA TTCGCCACCa tgaactggct atcgagttcc tcgggagttg tgctaactgc 3721 ttaccacccc agcggcaagg accaggtcgc tggggacagc catgtaaagg gaggcgacga 3781 agccacctcc agtcgcagat atggccaata caccataaac caagaaggct ccaccaaagt 3841 tcccgagagg cctccatttg atcgatcgag ttctcaggat tccttggatg aatcaatgga 3901 agcctattgg acagaactag aaaacatcaa gaggtcgaat gaaaatcgcc aagaaggtca 3961 agaggccata gttgtcaaag aacctgatga gggagaactg gaagaagaat ggctcaaaga 4021 agcaggtcta tccaatcttt ttggagagtc tattgatgac ccgcaggaaa gcattctgtt 4081 tttatctaca ctgacccgga ctcaggcagc agctgttcag aaacgggtgg aaacggtctc 4141 ccagaccttg aggaagaaaa acaaacagca ccacattcgt gacgtcagag acatatttgc 4201 tcaacagaga gaggcacaag aaaaacctcc agatgactca gacttgcggt cagtcagaac 4261 aaacgaaaac aaaggccaag gaaaagatga tcagccatct agtggggctg tcgatagcaa 4321 ggagcagatc tcacgggtgc ctgaggacac acctgcctct gaaacagaca tcaatctgga 4381 ggtgtcattt gcggagcaag ctgtcaatca gaaagagttc agtaaggaga ggacgcagaa 4441 gatcagcagc aacgactctt tgcccagttt cagactgcca aaagataaaa caggtacgac 4501 aaggattgga gacttggcac cccaagacat gaagaaagtt tgctctctat ccctgattga 4561 actcactgcc ctgtatgatg tgttgggctt ggaattcaaa caacagaaag ctgtgaaaat 4621 caaaaccaga gactctggtc tttttggtat tcctttaacg atattgttag aacaagatca 4681 aagaaaagtg cctggaacac gaataccctt gatctttcaa aaactgattt ctcgaattga 4741 agaaggcagt ctggaaaccg agggcctcct acggatacca ggagcagcca tgagaatcaa 4801 gaatctttgc caagaactag aagcgaaatt ttacgagggg actttcaatt gggaaagtgt 4861 caaacagcac gatgcggcca gcctgctgaa gctctttctg agagaattgc cccagccact 4921 gctgagcatg gagtatctca aagccttcca agctgtccag aatcttccaa ccagaaagga 4981 acagttgcag gctttgaacc tccttgtcat tcttctacct gacgcaaaca gagacacact 5041 gaaggccttg cttgaatttc tccaaagagt catagataac aaagagaaga acaagatgac 5101 agctgggaat gtagcaatgg tcatggcccc aaatctcttc atgtgccaca cgctgggctt 5161 gaagtccagt gaacagcgag aatttgaaat ggcagctggg acagcaaatg tcatgcactt 5221 attgattagg taccagaaaa ttctatggac aatccctaaa tttattgtaa tccaagtgag 5281 gaaacagaat attgagaatc aaaagaagga aaggaaagcc atgaagaaat tgctgaagaa 5341 aatggcttat gaccgggaaa aacatgaaaa gcaagataaa actgcaaatg gtgctgatgt 5401 tcctcaggga gtgattcgag tgcaagctcc ccatctttca aaagtttcca tggcaataca 5461 gctaactgaa gaactaaaag ccagcgatgt gcttgccagg tttctcagcc aagaaagtgg 5521 ggttgcccag acactcaaga aaggagaagt tttcctgtat gagattggag gaaatattgg 5581 ggaacgttgc ctcgatgacg acacacacat gaaggacttg taccaactga acccgaatgc 5641 cgagtgggtc ataaaatcca agccagtgGG ATCCatgGAC TACAAAGACC ATGACGGTGA 5701 TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGGCGG CCGCAAGGAT 5761 CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA 5821 GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG 5881 GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT 5941 AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGCT 6001 GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC CGCCCTACCT GAGGCCGCCA 6061 TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT GAACTGCGTC 6121 CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC CTTTGTCCGG CGCTCCCTTG 6181 GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG CTCAACTCTA 6241 CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC AAGCTGTGAC CGGCGCCTAC 6301 GCTAGAATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT 6361 ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC TCTCTCTAGC 6421 GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA 6481 CTCGTGGTGC TGGGCACTGC TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG 6541 ATCGGAAATG AGAACAGGGG CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC 6601 GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT 6661 GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAAATCA ACCTCTGGAT 6721 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 6781 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 6841 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 6901 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 6961 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 7021 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 7081 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC 7141 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 7201 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 7261 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGTA CCTTTAAGAC CAATGACTTA 7321 CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT 7381 TCACTCCCAA CGAAAATAAG ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA 7441 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 7501 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 7561 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT 7621 TATTATTCAG TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT 7681 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 7741 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 7801 CTGGCTCTAG CTATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 7861 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 7921 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GACTTTTGCA GAGACGGCCC AAATTCGTAA 7981 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA 8041 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA 8101 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA 8161 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG 8221 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 8281 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 8341 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 8401 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 8461 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 8521 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 8581 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 8641 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 8701 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 8761 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 8821 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 8881 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 8941 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 9001 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 9061 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 9121 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 9181 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 9241 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 9301 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 9361 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 9421 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 9481 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 9541 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 9601 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 9661 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 9721 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 9781 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 9841 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 9901 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 9961 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 10021 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 10081 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 10141 GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC 10201 TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG 10261 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA 10321 GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG 10381 AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC 10441 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT 10501 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG 10561 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT G

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.