pLV2-CMV-EGFP-MCS-IRES-Puro pl

pLV2-CMV-EGFP-MCS-IRES-Puro plasmid

PVT107155

pLV2-CMV-EGFP-MCS-IRES-Puro plasmid Information

Plasmid name: pLV2-CMV-EGFP-MCS-IRES-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-EGFP-MCS-IRES-Puro plasmid Description

pLV2-CMV-EGFP-MCS-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV2-CMV-EGFP-MCS-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggtg 2821 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 2881 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 2941 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 3001 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 3061 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 3121 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 3181 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 3241 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 3301 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 3361 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 3421 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 3481 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc 3541 agatctcgag GCGGCGGAGG GAGTGGAGGA GGGGGAAGCa ctagttctag agcggccgcg 3601 gatcccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 3661 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 3721 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 3781 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 3841 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 3901 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 3961 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 4021 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 4081 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 4141 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccacaag 4201 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 4261 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 4321 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 4381 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 4441 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 4501 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 4561 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 4621 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 4681 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 4741 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 4801 gcccggtgcc tagacgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat 4861 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 4921 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 4981 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 5041 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 5101 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5161 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5221 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5281 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 5341 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 5401 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat 5461 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga 5521 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc 5581 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc 5641 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga 5701 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta 5761 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag 5821 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg 5881 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt 5941 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag 6001 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc 6061 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 6121 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 6181 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 6241 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 6301 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta 6361 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6421 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6481 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6541 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6601 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6661 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6721 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6781 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6841 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6901 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6961 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7021 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7081 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7141 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7201 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 7261 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7321 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7381 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7441 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7501 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7561 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7621 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7681 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7741 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7801 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 7861 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7921 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7981 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8041 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8101 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8161 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8221 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8281 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8341 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8401 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8461 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8521 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat 8581 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 8641 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata 8701 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat 8761 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga 8821 aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt 8881 aaatatgtac tacaaactta gtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.