psiCHECK-Bdnf-3UTR plasmid

psiCHECK-Bdnf-3UTR plasmid

PVT111087

psiCHECK-Bdnf-3UTR plasmid Information

Plasmid name: psiCHECK-Bdnf(rat)-3UTR
Alias:
Gene lenght: 660
Host: Mammalian cells
Use(s): Signal report
Fragment type: UTR
Species: Rat
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc,Rluc
Promoter: SV40
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: hRluc-F3
Reverse primer:
Copy number: High
Induction:

psiCHECK-Bdnf-3UTR plasmid Description

psiCHECK-Bdnf-3UTR is a plasmid suitable for dual-luciferase reporting in mammalian cells.

psiCHECK-Bdnf-3UTR plasmid Sequence

1 AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT 61 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 241 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 301 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG 361 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 421 AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA 481 CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT 541 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC 601 ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA 661 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG 721 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG 781 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT 841 CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC 901 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT 961 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC 1021 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC 1081 TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG 1141 ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA 1201 GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG 1261 ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG 1321 GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC 1381 AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT 1441 CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA 1501 GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG 1561 GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC 1621 GAGCAGTAAT TCTAGGCGAT CGCTCGAGtg gatttatgtt gtatagatta tattgagaca 1681 aaaattatct atttgtatat atacataaca gggtaaatta ttcagttaag aaaaagataa 1741 ttttatgaac tgcatgtata aatgaagttt atacagtaca gtggttctac aatctattta 1801 ttggacatat ccatgaccag aaagaaacag tcatttgcgc acaactttaa aagtctgcat 1861 tacattcctc gataatgttg tggtttgttg ccgttgccaa gaattgaaaa caaaaagtta 1921 aaaaaaataa taaattgcat gctgctttaa ttgtgaattg ataataaact gtccctcttt 1981 cagaaaacag acaaaaaaac aaaaaacaaa aaaaagcaaa aacaaaaatt tgaaccaaaa 2041 cattccgttt acattttaga cactaagtat cttcgttctt gttagtactc tgttctactg 2101 ctttcaactt ctcatagcgt tggaattaaa actatgtcaa ggtgctgttg tcattgcttt 2161 actggcttag gggatgggga acgggagggg tagatttctg tttgttttgt gttttatttc 2221 gtttgtttgt ttgttttgtt tttttagttc cacccggagt agggatggag aaaatttctt 2281 cactctccat tctggttgat aaagcgttGC GGCCGCTGGC CGCAATAAAA TATCTTTATT 2341 TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAGGATCTA AATGAGTCTT CGGACCTCGC 2401 GGGGGCCGCT TAAGCGGTGG TTAGGGTTTG TCTGACGCGG GGGGAGGGGG AAGGAACGAA 2461 ACACTCTCAT TCGGAGGCGG CTCGGGGTTT GGTCTTGGTG GCCACGGGCA CGCAGAAGAG 2521 CGCCGCGATC CTCTTAAGCA CCCCCCCGCC CTCCGTGGAG GCGGGGGTTT GGTCGGCGGG 2581 TGGTAACTGG CGGGCCGCTG ACTCGGGCGG GTCGCGCGCC CCAGAGTGTG ACCTTTTCGG 2641 TCTGCTCGCA GACCCCCGGG CGGCGCCGCC GCGGCGGCGA CGGGCTCGCT GGGTCCTAGG 2701 CTCCATGGGG ACCGTATACG TGGACAGGCT CTGGAGCATC CGCACGACTG CGGTGATATT 2761 ACCGGAGACC TTCTGCGGGA CGAGCCGGGT CACGCGGCTG ACGCGGAGCG TCCGTTGGGC 2821 GACAAACACC AGGACGGGGC ACAGGTACAC TATCTTGTCA CCCGGAGGCG CGAGGGACTG 2881 CAGGAGCTTC AGGGAGTGGC GCAGCTGCTT CATCCCCGTG GCCCGTTGCT CGCGTTTGCT 2941 GGCGGTGTCC CCGGAAGAAA TATATTTGCA TGTCTTTAGT TCTATGATGA CACAAACCCC 3001 GCCCAGCGTC TTGTCATTGG CGAATTCGAA CACGCAGATG CAGTCGGGGC GGCGCGGTCC 3061 CAGGTCCACT TCGCATATTA AGGTGACGCG TGTGGCCTCG AACACCGAGC GACCCTGCAG 3121 CGACCCGCTT AAAAGCTTGG CATTCCGGTA CTGTTGGTAA AGCCACCATG GCCGATGCTA 3181 AGAACATTAA GAAGGGCCCT GCTCCCTTCT ACCCTCTGGA GGATGGCACC GCTGGCGAGC 3241 AGCTGCACAA GGCCATGAAG AGGTATGCCC TGGTGCCTGG CACCATTGCC TTCACCGATG 3301 CCCACATTGA GGTGGACATC ACCTATGCCG AGTACTTCGA GATGTCTGTG CGCCTGGCCG 3361 AGGCCATGAA GAGGTACGGC CTGAACACCA ACCACCGCAT CGTGGTGTGC TCTGAGAACT 3421 CTCTGCAGTT CTTCATGCCA GTGCTGGGCG CCCTGTTCAT CGGAGTGGCC GTGGCCCCTG 3481 CTAACGACAT TTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATTTCT CAGCCTACCG 3541 TGGTGTTCGT GTCTAAGAAG GGCCTGCAGA AGATCCTGAA CGTGCAGAAG AAGCTGCCTA 3601 TCATCCAGAA GATCATCATC ATGGACTCTA AGACCGACTA CCAGGGCTTC CAGAGCATGT 3661 ACACATTCGT GACATCTCAT CTGCCTCCTG GCTTCAACGA GTACGACTTC GTGCCAGAGT 3721 CTTTCGACAG GGACAAAACC ATTGCCCTGA TCATGAACAG CTCTGGGTCT ACCGGCCTGC 3781 CTAAGGGCGT GGCCCTGCCT CATCGCACCG CCTGTGTGCG CTTCTCTCAC GCCCGCGACC 3841 CTATTTTCGG CAACCAGATC ATCCCCGACA CCGCTATTCT GAGCGTGGTG CCATTCCACC 3901 ACGGCTTCGG CATGTTCACC ACCCTGGGCT ACCTGATTTG CGGCTTTCGG GTGGTGCTGA 3961 TGTACCGCTT CGAGGAGGAG CTGTTCCTGC GCAGCCTGCA AGACTACAAA ATTCAGTCTG 4021 CCCTGCTGGT GCCAACCCTG TTCAGCTTCT TCGCTAAGAG CACCCTGATC GACAAGTACG 4081 ACCTGTCTAA CCTGCACGAG ATTGCCTCTG GCGGCGCCCC ACTGTCTAAG GAGGTGGGCG 4141 AAGCCGTGGC CAAGCGCTTT CATCTGCCAG GCATCCGCCA GGGCTACGGC CTGACCGAGA 4201 CAACCAGCGC CATTCTGATT ACCCCAGAGG GCGACGACAA GCCTGGCGCC GTGGGCAAGG 4261 TGGTGCCATT CTTCGAGGCC AAGGTGGTGG ACCTGGACAC CGGCAAGACC CTGGGAGTGA 4321 ACCAGCGCGG CGAGCTGTGT GTGCGCGGCC CTATGATTAT GTCCGGCTAC GTGAATAACC 4381 CTGAGGCCAC AAACGCCCTG ATCGACAAGG ACGGCTGGCT GCACTCTGGC GACATTGCCT 4441 ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGCCT GAAGTCTCTG ATCAAGTACA 4501 AGGGCTACCA GGTGGCCCCA GCCGAGCTGG AGTCTATCCT GCTGCAGCAC CCTAACATTT 4561 TCGACGCCGG AGTGGCCGGC CTGCCCGACG ACGATGCCGG CGAGCTGCCT GCCGCCGTCG 4621 TCGTGCTGGA ACACGGCAAG ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC 4681 AGGTGACAAC CGCCAAGAAG CTGCGCGGCG GAGTGGTGTT CGTGGACGAG GTGCCCAAGG 4741 GCCTGACCGG CAAGCTGGAC GCCCGCAAGA TCCGCGAGAT CCTGATCAAG GCTAAGAAAG 4801 GCGGCAAGAT CGCCGTGTAA TAATTCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA 4861 TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT 4921 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC 4981 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG 5041 TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCAGGTGGCA 5101 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA 5161 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA 5221 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 5281 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 5341 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC 5401 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT 5461 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT 5521 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 5581 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA 5641 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC 5701 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 5761 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 5821 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 5881 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 5941 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 6001 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 6061 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 6121 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 6181 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 6241 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 6301 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 6361 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT 6421 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 6481 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 6541 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 6601 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA 6661 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 6721 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 6781 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 6841 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 6901 TGGCTCGAC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.