pLV2-CMV-MCS-FLAG-T2A-mCherry

pLV2-CMV-MCS-FLAG-T2A-mCherry plasmid

PVT100548

pLV2-CMV-MCS-FLAG-T2A-mCherry plasmid Information

Plasmid name: pLV2-CMV-MCS-FLAG-T2A-mCherry
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: PGK-R
Copy number: High
Induction:

pLV2-CMV-MCS-FLAG-T2A-mCherry plasmid Description

pLV2-CMV-MCS-FLAG-T2A-mCherry It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-MCS-FLAG-T2A-mCherry plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGGAG GAGGGGGAAG Cgactacaag gacgacgatg acaagggcag 2881 cggtgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggcccttc 2941 cggaatggag agcgacgaga gcggcctggt gagcaagggc gaggaggata acatggccat 3001 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 3061 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 3121 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 3181 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 3241 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 3301 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 3361 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 3421 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 3481 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 3541 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 3601 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 3661 cgagctgtac aagtagggat cccgcgactc tagataattc taccgggtag gggaggcgct 3721 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac 3781 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt 3841 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc 3901 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt 3961 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata 4021 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg 4081 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt 4141 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct 4201 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc 4261 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 4321 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 4381 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 4441 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 4501 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 4561 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 4621 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 4681 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 4741 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 4801 atgacccgca agcccggtgc ctgaccgcgt ctggaacaat caacctctgg attacaaaat 4861 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 4921 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 4981 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5041 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5101 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5161 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5221 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5281 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5341 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 5401 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa 5461 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa 5521 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg 5581 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg 5641 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc 5701 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt 5761 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga 5821 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa 5881 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat 5941 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac 6001 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata 6061 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga 6121 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6181 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6241 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6301 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac 6361 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 6421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6541 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 6601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 6841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 6961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7201 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 7261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 7501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 7561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 7621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 7681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 7741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 7801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 7861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 7921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 7981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 8341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 8401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 8461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 8521 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt 8581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 8641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 8701 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt 8761 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt 8821 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa 8881 attgtatttg ttaaatatgt actacaaact tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.