pCAG-HA-Helz2 plasmid

pCAG-HA-Helz2 plasmid

PVT111040

pCAG-HA-Helz2 plasmid Information

Plasmid name: pCAG-HA-Helz2(mouse)
Alias: NM_183162.2;mKIAA1769; mPDIP1; PDIP1; Pric285
Gene lenght: 8844
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCAG-HA-Helz2 plasmid Description

pCAG-HA-Helz2 It is a plasmid suitable for gene expression in mammalian cells.

pCAG-HA-Helz2 plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGC CACCATGTAC 1741 CCATACGATG TTCCAGATTA CGCTGAATTC atggcatccg tgggttgcag tctgaggtcg 1801 gccagcacat ccgccaccaa cgggccatcc ttagccggac tctgtgctaa ggtagacctt 1861 tacctgggct gctcccgctg cacccagtgc ctcaatgaga gtacctacat tctgcgtgag 1921 gtagagcaca cctgccccag ggagatcttg ctggcccgct tcaagcaagc agcagagagc 1981 aagatctggc gtaaggtggg ccgcaggcct agcttcccaa cgcctatgcg ctatcaagtc 2041 tgtcactact ataggccagg gttgggctgt aggcgtcact ggaatagatg cacctttgcc 2101 cgaagccctg aggaggcact ggtctggaca tttgagctca agaacaacct tccccgattg 2161 aaactgaaag aggctgtgca gggcaccaga gccccagata ggctgcagac cccagctgac 2221 accatccgag cagagtttgg tggccacttc caactactct gtgccatctg cttcacgtgc 2281 tgtcccccgt gcctctgccc cgtggatccc aggggccatt gccctaagca ccaaatctgc 2341 cccacacttc tcatccatgt tatagttgag ggcctaaagc gacaattcgt ggaggtgcgg 2401 ccactgccac aaagaagaca tcctttaaac tactgcatgt atgtgggccg tggggtacca 2461 tgccgccatg gggcctcacg ctgtgagtat gcacacagtg ctgtggagat ggctgtgtgg 2521 aaggctgagc agttggatgg gcttcagaga ggagatctgc tcacatatcc tctctttggg 2581 gaaaacaaat ggaaagccag ccctaaccct aaccctcctg tgaccaaact gtactgccat 2641 gcctgcctgg tcacctgtaa ttctcaagag gcatttgaga accactgctc ctccctggag 2701 cacgcacaga tggtggcctt tgaccaggct gtgccctgga aacaccgtgc cccaccaatg 2761 ggactctcca agttcgatct ctgccctagg cctgatctct gtgagcacgg agaggtctgc 2821 atcaaggcac actcaaaaca ggagttacag gagtgggttc agcgggcaca agacatggaa 2881 ctgcgagaac aggccgcctg gcaggatggg ctggtacctt accaggcacg acttctagcg 2941 gaataccagc gcagcagcaa agaagtatca gtgatggctg agaccatccg tggagtgtct 3001 gtcacttgcc acccaccacc ggtgcatcag gcccaggaga agatccagca ccagtgggtg 3061 ttcaccatcc actctgagga tcccttgcta catgtggccc tactcaatca agagcctgga 3121 gcagctttct cactggtggc ccccagtctc ccaccacacc agctctatgc acagggaaag 3181 cacttctgtg tacaaagctc cccagcccaa tacaaggtag gggtgcttgt gcaggctgtc 3241 gccttcggca gctttgagca gtgggtggtc tttgactttg gtcgcaggcc agtgcttctc 3301 cagaagctga agttgcagct aggccagaca cacagccagg gcctaaatgg gaagccagca 3361 cccagccacc cccaggagct ggaatgctgg cacacaggca accgccacgt ggtgcttgag 3421 gtagattgga cacccgagca ggaagccctg atggccaagt acaagttacc ctccctggcc 3481 ctggagttca atcagattgt tccggactgg gggcccatct ctcgttcgaa ctaccgacag 3541 aggatgcaca aatttctcta tgaggaggaa gcagcccagc agcagctagt agccaagctg 3601 gccatgaagg gccaggtatc cctgaagaca gcactggaga caccagcact cggcatgctc 3661 ttcgcaccac caggggccct ctatgccaag gtccccttcc actcctctct gttgccagac 3721 actgatcagg gcttcctgtt gagccgagca gtcagcacag cccttgtggc tcccgtgcct 3781 gcgcctaaca gtacagtata ccaagtgcgg ctggaggcac gggccagttc agaccatgcg 3841 ctgtggctgc tgttaccagc acgatgctgt atggctttgg ggctgcaggc ccaagacagc 3901 cccatcttgg aggtgcagtt ccagattgac cctatgacct tccggttctg gcatcaggca 3961 gtggatgcac tgctcgagga gcacctggtg gttcctgacc tgcctgcctg caccctgccc 4021 cacccctggc ccaccccacc ctcatttcgt ggcaatcaca aacagaagct ggctgtgggg 4081 ctcattgcag gcagaagacc cgagggcaca aagcacatcc caccactgct catctatggc 4141 ccctttggca ccggcaagac gtacacattg gccatggccg cacttgaggt tgtccagcag 4201 ccccacacca aggtgctcat ctgcacacac accaacagtg ctgcagacat ctatatccgg 4261 gagtatttcc acgactacgt cagcagtggc catccagagg ccactccact cagggtgatg 4321 tatgcagatc gtcctccaag acagactgat ccaaccacgt tgcagtactg ctgcctaact 4381 gaggaccgcc aggcctttcg cccacccaca ggcccagagc tggtacatca ccgtttagtg 4441 gtcaccacca cttctcaggc tcgtgagctc caggtgccag cgggcttctt ctcccacatt 4501 ttcatcgatg aggcagccca aatgctggag tgtgaggccc ttatcccact ctcctatgcc 4561 ttgtcactaa cccgtgtagt actggcaggg gaccacatgc aggtcacacc taggctcttc 4621 agcgtgccta gggacaagtc agccaggcac acgctgctgc accgcctctt cctgtactac 4681 cagcaggagg cacacaagat cgctcagcag agtcgcatca tcttccatga gaactaccgg 4741 tcaactgcag ccatcatcaa cttcgtctcc catcatttct atttagccaa gggtaatccc 4801 atccaggcca gtggcaaagt cccacgtcac ccccaacact accctctcat gttctgccat 4861 gtggcaggaa gccctgagca ggacatgtcc atgacatcct ggcttaactc ggctgaggtc 4921 acccaggtgg tggagaaggt gcgggagatc tataacacct ggcctcactg ctggggaccc 4981 cgggagcaga gacacatctg tgctgtctct catggtgccc aggttagtgc gctgagacag 5041 gagctaagaa ggaggaacct tggtgaggtg tctgtgggca gctttgagat cctgccaggg 5101 cgtgagttcc gggtggtggt actgagctct gtccacaacc gcaatagtct gcttagccca 5161 ggggcaccaa cctcagagtt cttcactgag cctcgagtac tgaacacggt catgactcgt 5221 gcccagtctc agctggtggc tgtgggagat gctgtggccc tctgctcctc tggagcctgc 5281 agaaatctct ggagaagctt catccgcgag tgcatagaac accacagcgc cttccctgag 5341 gagctgtcac tggaacagat tgagcagggt gtggcccaga gacagaactg ggcctcattg 5401 acactcaaag ctaggggccc agagacagag caaaaatcta tggcccaggg cccacaaagg 5461 ctcatagctg aagggaccat ggtcacggtg aaggcagaga ccagagccga ggcagcagcc 5521 aaggcacaga ctgcggcagt cgcagcagaa gacacagcaa gcgggaactc agcctctaga 5581 gatgcagcag cagaagtgtc caccctggaa ggggggatgt ctgaggaaga ttctgagtct 5641 gacttctggc cctctgactg ggagctcaat gctgatgacg ccatcctgaa ggaacttctc 5701 gacgagagcc agcaagtgac tgtgactgtt agggaggacg gacttctgga caccgtggtt 5761 tgctctgcac cacagaaggc ccgagagtac acaaacctac cctcatctgt gctgtggaag 5821 ttcctgcgct caaactccaa gcaattccgc cgctgcagct tcctacagga gacctttgag 5881 agagcattgg ccactccgct ggacgacatg gcctctagcc ctatccaggt caggggccgt 5941 ctgaactgtg ggatggcatt cacgggggat gaggtgctag tacagatcct gggtccggca 6001 ggagatgaca ggtgcgtccc tgggagcctg cagggtcgtg tgatgggtgt gttgaagagg 6061 aggaggcacg aattggcctt tgtgtgtcgg atggatgagt gggacccccg catcatgatc 6121 cctatcaacg gttctgtgac taagattttt gtggcagaga tgaaggaccc acagcaagtt 6181 cccatccacc gcctcattca aggccaggtg cagcgtgtga ggcatgagac cctcaagcct 6241 gaggatcgga gcacccggct tttctgggtc cgtattgtcc tttggcggga acgtttctac 6301 tatccactag gcattgtcct ggaggtgctg cccaaggcca tcacctggga gcagggcctt 6361 tatatccttg acctagagca tggcctgaag gcccacacac cagacccagc ctcagtttcc 6421 aaggctctgc agagataccg ctcagagcta aacacggccg ctggtcaccg agaggactac 6481 cgccacttcc tgaccttcac tgtggacccc cagggtgcct gcaaccttga cgacgctctt 6541 agtgtccgag acctaggccc agtgtacgag gtggcagtgc atattgctga tgtggccagc 6601 ttggtgccca aggatggggc cctggacgtg gaagcccgcc agcagggaac tgtgttctat 6661 gcccccaata gggagccagt actcatgctg cccgccagct tgtgccagga tgcactcagc 6721 cttctccctg gtcaggatcg cctcgccatc tctctcttcc tcaccatgga gaaaggtggt 6781 ggccagatca agagcctgcg atttgcaccc tccataatcc gctctgaccg ccagctgtcg 6841 tatgaggaag ccgaagaact gatcaagaga caccctgggg ctggcctgga gcttccagcc 6901 cacctggact ctgtggaggc ctgtgttgta gctgcctgct acttctcttg gatgttacgt 6961 cgccagcgcc tctcagctgc ctgttactat gagccgccag acgaggacag tgtgctgggc 7021 ttccgcacag ctcacatcat ggtccaagag tacatgatcc agtttaacag ccatgtggct 7081 gaattccttg tgagcaacaa gcacacacag acactcacac cactcaggtg gcagcccaca 7141 cccagtaggc agcagcttga cagtgttttc aagaagtaca gaggcctggt gcctctgtcc 7201 ctgcacctgt gccaccactc gaacactgac tataccccca acaagcagct gcatctcctg 7261 acttccctct ggaaacaagt gcagcttgca gctggtactc aggattacag ccagatggtg 7321 gaccttattg ctgcagatga catgcaccct tcactggctc ctgcttgcct ggacttgcga 7381 agggctctag gccgctctgt ttttggtcgc tccagccagg gtaagcagca acctgctgtt 7441 caccactcgc tacaggtgga ttggtacaca tgggcaacct cacccatccg taggtatcta 7501 gatgtggtgc tgcagcggct gatcctgctg gcattgggcc acaggggctc tacatattct 7561 aacagggaca ttgatgggct ctgcctggac ttcagtcgcc agtatgcaag tgcccagagc 7621 taccaacgga gggcctacag cctgcacctg gccatccagc tcaagagcca gcctcaaaac 7681 aagctgggct tcgtagtgga tgtcgagatg ggtgcccgct gcttcaaagt gcttttccct 7741 ataaaccggg aaacgctgcc cgatccctgc ccaattcact accattccct gcagttggct 7801 gagcaccccc aggagctagt gagccagaca ggcgtgcggc ttgtatggcg gcgccgcatg 7861 tactcggtgc aggcgtccaa gctgccttta ccacttctgg gcacgagtct tgatccacac 7921 acccagactg tagatgcagc cctgtggatg aagctactga tgctgctgaa ggagcagcgc 7981 tggcctgaga tagctgccct catccaggag caagataagc gttttcatcc aagggagaaa 8041 gtaaagatcc accagagccg ctgtggacac tttgtagagg tggtctacga gctgggcagt 8101 ggagataccc tgcaggttca gcttggttcc agcctgcagc ggggtttcct ggcaccaacc 8161 ctgaagctgt ggactgtggt ccctggcttc agcctctgtc tggagcacat ggaacggcca 8221 ggtgactgct tttcaagcca tgtgcaccag gctctgcaag accagtacct tcaagtcggc 8281 gagtactcag gtgcctgggg gccacgctgc gccctggagt cgctcaccaa tgctgttaca 8341 gaaaacgact ccattgtgct gcacgatgtg catatctctt gggacacatc ccaggggcag 8401 ttgcagggca ccttccagct ggaagctgct tttctccagg agaaatgcat caacatccac 8461 tttggctgtt gctacctctg cattcggctg gaggggctcc ctctccccct agatagctca 8521 ctccctgggc ctagtggcct tggccccttc ctgaacattg accccaatac atatacctgg 8581 gtggcccatg ggctgtctgg tgactgggac catgaactgg ctggtggtga ctgggaccag 8641 gagaatgtgg atgatcggca ggaggcgccc aagcaggtat attttctcat ccatcacatg 8701 accatggaga aggttccaga agaggtgctg agacctagcg ctcggttcac tgtggaagtg 8761 ctgtctaagc agcttcctga cctccgcaag gaagaagcag tgcgtcaact gaaaacagca 8821 tccccattgg tcattagcat cgccttgggc ctgcctatcc ctgagatccg ctggccaata 8881 tctgggcccc gtcgcttagt atctgagctc cgttggccaa tacccgggcc ccgtcgccca 8941 gtatctgagc cccatcgccc aatgtccggg ccctgtggcc caatatctga accgtgtcgc 9001 tcaatccccg agccctgccg cggcaactgg cctcgccagc attcttttca caaggcatct 9061 accagcaggt tcttggagcg gcagaactac aacatccctg ctggacatca taagctgaac 9121 cagagccagg acagggctgt caggagcgct ctgcagaaac agttcacagt catccagggc 9181 ccaccaggca ctgggaagac cgttgtgggc ttccacattg tgtactggtt ccacaggtca 9241 aaccaggagc agatgccaac tgacagcagc ccgagtggag aggagcagct ggggggccca 9301 tgtgtcttat actgtggacc ctccaacaag tctgtggatg tcctgggagg actgctcctg 9361 aggaggaaga cagagatgaa gcccctgcgt gtatatgggg agcaggcaga ggccactgaa 9421 ttcccgctgc caggagtgag caacaggagt ctgtttggca agacctctca ggaaggaaga 9481 ccaaatcaga gcctcaggag cattaccctt caccaccgga tccgccaggc ccccaaccca 9541 tatgcagcag agatcagaaa atttgatgcc cagcttcggg aagggaaaat attctcaaag 9601 gaggatctta gagtgtacag gagagtcttg gggaaggcac gcaaacatga actggagcgg 9661 cactcggtca tcctgtgcac gtgctcctgt gcggcatcaa agagcctgaa aattctgaat 9721 gttcgacaga tccttatcga cgaggcaggc atggccactg agccagagac ccttatcccc 9781 ttggtgtgct tctcaaagac ggttgagaag gtggttctgc ttggagacca caagcagctt 9841 cggcctgtgg tcaagagtga acagctgcag agtctgggga tggatcggtc tctctttgag 9901 aggtaccaca gggatgccat catgctggac acacagtacc gtatgcataa ggacatctgt 9961 tccttcccct ccgtggagtt ctatggggga aagctaaaga cctggtctga cctgaggcgt 10021 ctgcctagta tcctgggtca cactggcaag ccgagctgtt ccgtcatctt cggctctgtg 10081 cagggccatg agcagaagct gctggtgtcc accgaggatg gcaatgagaa ctctagggcc 10141 aacccagagg aagtcacaca ggtggtccgc atcataaagc agttgaccct ggatcggacg 10201 gtggatccca aagatattgc tgtccttaca ccctacaatg cacaggctgc tgcaatcagt 10261 aggggcctca tgcaaagagg agtcaccgga gtgactgtga cctctattac caagagccag 10321 gggagtgagt ggcgctatgt gatagtgagt accgtccgaa catgccccag gagtgatgtg 10381 gaccagcggc caacaaagag ctggctcaag aagtttctgg gctttgttgt ggacccccac 10441 caagtaaacg tggccatcac ccgggcccag gaggcgttgt gcatcattgg ggaccatcta 10501 ctgctacgct gctgcccact ctggcaccgt ctcctagact tctgtgaggc tcaacacagc 10561 ctggtgtccg ctgagaaggt gagggtccag agaaaatctg ctctgtcttc ttgaCTCGAG 10621 CTAGCAGATC TTAATTAATT AAGGATCTTT TTCCCTCTGC CAAAAATTAT GGGGACATCA 10681 TGAAGCCCCT TGAGCATCTG ACTTCTGGCT AATAAAGGAA ATTTATTTTC ATTGCAATAG 10741 TGTGTTGGAA TTTTTTGTGT CTCTCACTCG GAAGGACATA TGGGAGGGCA AATCATTTAA 10801 AACATCAGAA TGAGTATTTG GTTTAGAGTT TGGCAACATA TGCCCATATG CTGGCTGCCA 10861 TGAACAAAGG TTGGCTATAA AGAGGTCATC AGTATATGAA ACAGCCCCCT GCTGTCCATT 10921 CCTTATTCCA TAGAAAAGCC TTGACTTGAG GTTAGATTTT TTTTATATTT TGTTTTGTGT 10981 TATTTTTTTC TTTAACATCC CTAAAATTTT CCTTACATGT TTTACTAGCC AGATTTTTCC 11041 TCCTCTCCTG ACTACTCCCA GTCATAGCTG TCCCTCTTCT CTTATGGAGA TCCCTCGACC 11101 TGCAGCCCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 11161 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 11221 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 11281 CTGTCGTGCC AGCGGATCCG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC 11341 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 11401 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT 11461 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT AACTTGTTTA TTGCAGCTTA 11521 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 11581 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCCGCTG 11641 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 11701 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 11761 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGATAA CGCAGGAAAG AACATGTGAG 11821 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 11881 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 11941 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 12001 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 12061 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 12121 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 12181 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 12241 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 12301 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 12361 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 12421 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 12481 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 12541 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 12601 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 12661 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 12721 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 12781 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 12841 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 12901 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 12961 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 13021 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 13081 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 13141 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 13201 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 13261 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 13321 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 13381 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 13441 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 13501 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 13561 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 13621 CTGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.