pTac-GST-MAF plasmid

pTac-GST-MAF plasmid

PVT110555

pTac-GST-MAF plasmid Information

Plasmid name: pTac-GST-MAF(human-gc-opt)
Alias: NM_005360.5;AYGRP; c-MAF; CCA4; CTRCT21
Gene lenght: 1209
Host: E.coli
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F1
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-MAF plasmid Description

pTac-GST-MAF Plasmid suitable for gene expression in Escherichia coli.

pTac-GST-MAF plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccATGGC ATCAGAACTG GCAATGAGCA 961 ACTCCGACCT GCCCACCAGT CCCCTGGCCA TGGAATATGT TAATGACTTC GATCTGATGA 1021 AGTTTGAAGT GAAAAAGGAA CCGGTGGAGA CCGACCGCAT CATCAGCCAG TGCGGCCGTC 1081 TCATCGCCGG GGGCTCGCTG TCCTCCACCC CCATGAGCAC GCCGTGCAGC TCGGTGCCCC 1141 CTTCCCCCAG CTTCTCGGCG CCCAGCCCGG GCTCGGGCAG CGAGCAGAAG GCGCACCTGG 1201 AAGACTACTA CTGGATGACC GGCTACCCGC AGCAGCTGAA CCCCGAGGCG CTGGGCTTCA 1261 GCCCCGAGGA CGCGGTCGAG GCGCTCATCA GCAACAGCCA CCAGCTCCAG GGCGGCTTCG 1321 ATGGCTACGC GCGCGGGGCG CAGCAGCTGG CCGCGGCGGC CGGGGCCGGT GCCGGCGCCT 1381 CCTTGGGCGG CAGCGGCGAG GAGATGGGCC CCGCCGCCGC CGTGGTGTCC GCCGTGATCG 1441 CCGCGGCCGC CGCGCAGAGC GGCGCGGGCC CGCACTACCA CCACCACCAC CACCACGCCG 1501 CCGGCCATCA TCATCATCCA ACTGCTGGTG CACCAGGAGC TGCTGGTAGT GCTGCAGCAT 1561 CTGCAGGTGG TGCTGGAGGT GCAGGTGGTG GTGGACCAGC AAGTGCTGGA GGAGGTGGAG 1621 GTGGTGGTGG AGGAGGAGGT GGCGGTGGAG CAGCTGGAGC AGGGGGTGCC CTGCACCCGC 1681 ATCACGCTGC CGGAGGCCTG CACTTCGACG ACCGCTTCTC CGACGAGCAG CTGGTGACCA 1741 TGTCTGTGCG CGAGCTGAAC CGGCAGCTGC GCGGGGTCAG CAAGGAGGAG GTGATCCGGC 1801 TGAAGCAGAA GAGGCGGACC CTGAAAAACC GCGGCTATGC CCAGTCCTGC CGCTTCAAGA 1861 GGGTGCAGCA GAGACACGTC CTGGAGTCGG AGAAGAACCA GCTGCTGCAG CAAGTCGACC 1921 ACCTCAAGCA GGAGATCTCC AGGCTGGTGC GCGAGAGGGA CGCGTACAAG GAGAAATACG 1981 AGAAGTTGGT GAGCAGCGGC TTCCGAGAAA ACGGCTCGAG CAGCGACAAC CCGTCCTCTC 2041 CCGAGTTTTT CATAACTGAG CCCACTCGCA AGTTGGAGCC ATCAGTGGGA TACGCCACAT 2101 TTTGGAAGCC CCAGCATCGT GTACTTACCA GTGTGTTCAC AAAAtgactc gagcggccgc 2161 atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 2221 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 2281 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 2341 gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata cgcctatttt 2401 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 2461 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 2521 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 2581 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 2641 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 2701 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 2761 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg 2821 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 2881 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 2941 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 3001 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 3061 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa 3121 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 3181 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 3241 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 3301 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 3361 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 3421 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 3481 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 3541 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 3601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 3661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 3721 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 3781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 4021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 4141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 4201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 4261 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 4321 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga 4381 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat tccgacacca 4441 tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca 4501 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 4561 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 4621 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 4681 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 4741 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 4801 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 4861 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 4921 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 4981 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 5041 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 5101 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 5161 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 5221 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 5281 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg 5341 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 5401 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 5461 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 5521 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 5581 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 5641 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 5701 cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg 5761 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 5821 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 5881 gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct 5941 ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg 6001 ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt 6061 tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct 6121 acaggaaggc cagacgcgaa ttatttttga tggcgttgga att

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.