pTac-GST-CCDC86 plasmid

pTac-GST-CCDC86 plasmid

PVT110491

pTac-GST-CCDC86 plasmid Information

Plasmid name: pTac-GST-CCDC86(human)
Alias: NM_024098.4;Cgr1
Gene lenght: 1083
Host: E.coli
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F1
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-CCDC86 plasmid Description

pTac-GST-CCDC86 Plasmid suitable for gene expression in Escherichia coli.

pTac-GST-CCDC86 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatgga tacaccgtta aggcgcagcc 961 gacggctggg aggcctaagg cccgaatccc ccgagagcct cacctcagtt tcgcggacga 1021 gacgggccct tgtggagttc gagtcgaacc cagaagaaac gagggagccc gggtctcctc 1081 cgagtgtgca gcgggctggc ctggggtccc ccgaaaggcc gccgaagaca agcccaggat 1141 caccccgtct gcagcagggt gcaggcttgg agtcacccca agggcagcca gagccaggcg 1201 cagcgtcccc ccagcgtcag caagacctac acctggagtc gcctcaaaga cagccagagt 1261 acagtcctga atccccacga tgtcagccga agccaagtga ggaggcacca aagtgttctc 1321 aggaccaggg agtactggcc tcggagttgg cccagaataa ggaggagctg accccggggg 1381 ccccccagca tcagctaccg ccggtcccag gatcaccaga gccttacccc ggtcagcaag 1441 ctcccggtcc ggagccctct cagccactac tggagctgac acccagggca cctggctccc 1501 cccggggtca gcatgagccg agcaagccac ctccagctgg ggagacggtg acaggcggct 1561 tcggggcaaa gaagcgaaaa ggttcttcat cccaggcccc agcgtccaag aagttgaata 1621 aagaggagct tcctgtaatc ccgaagggga agcccaaatc ggggcgagtg tggaaggacc 1681 gctccaagaa aagattctcc cagatgcttc aggacaagcc cctgcgcaca tcgtggcagc 1741 ggaagatgaa ggaacgacag gagaggaagc tggccaagga ctttgcccgt cacctggagg 1801 aggagaagga gaggcgccgc caggagaaga aacagcgccg ggctgagaac ctgaaacgcc 1861 gcctggagaa tgagcggaag gcagaggtcg tccaagtgat ccgaaacccc gccaagctca 1921 agcgggcaaa gaagaagcag ctgcgctcca ttgagaagcg ggacaccctg gccctgctgc 1981 agaagcagcc gccccagcag ccggcagcca agatctgact cgagcggccg catcgtgact 2041 gactgacgat ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 2101 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 2161 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 2221 gcggagtgta taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta 2281 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 2341 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 2401 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 2461 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 2521 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 2581 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 2641 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag 2701 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 2761 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 2821 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 2881 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 2941 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa 3001 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 3061 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 3121 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 3181 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 3241 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 3301 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 3361 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 3421 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 3481 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 3541 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 3601 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 3661 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 3721 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 3781 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 3841 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 3901 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 3961 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 4021 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 4081 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 4141 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 4201 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt 4261 ttctccttac gcatctgtgc ggtatttcac accgcataaa ttccgacacc atcgaatggt 4321 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga 4381 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg 4441 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag 4501 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac 4561 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg 4621 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag 4681 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca 4741 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct 4801 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta 4861 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc 4921 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg 4981 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact 5041 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca 5101 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt 5161 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct 5221 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca 5281 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc 5341 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc 5401 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 5461 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 5521 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 5581 aacagctatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga 5641 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 5701 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 5761 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga 5821 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc 5881 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga 5941 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg 6001 ccagacgcga attatttttg atggcgttgg aatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.