pT7-GST-RNF166 plasmid

pT7-GST-RNF166 plasmid

PVT110350

pT7-GST-RNF166 plasmid Information

Plasmid name: pT7-GST-RNF166(human)
Alias: NM_178841.4
Gene lenght: 711
Host: E.coli
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F1
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-GST-RNF166 plasmid Description

pT7-GST-RNF166 Plasmid suitable for gene expression in Escherichia coli.

pT7-GST-RNF166 plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 1141 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 1741 actggcttca gcagagcgca gataccaaat actgtTcttc tagtgtagcc gtagttaggc 1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 2401 cgccCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGgatctcg 2461 atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc tctagaTCAC 2521 ACAGGAAACA GTATTCATGT CCCCTATACT AGGTTATTGG AAAATTAAGG GCCTTGTGCA 2581 ACCCACTCGA CTTCTTTTGG AATATCTTGA AGAAAAATAT GAAGAGCATT TGTATGAGCG 2641 CGATGAAGGT GATAAATGGC GAAACAAAAA GTTTGAATTG GGTTTGGAGT TTCCCAATCT 2701 TCCTTATTAT ATTGATGGTG ATGTTAAATT AACACAGTCT ATGGCCATCA TACGTTATAT 2761 AGCTGACAAG CACAACATGT TGGGTGGTTG TCCAAAAGAG CGTGCAGAGA TTTCAATGCT 2821 TGAAGGAGCG GTTTTGGATA TTAGATACGG TGTTTCGAGA ATTGCATATA GTAAAGACTT 2881 TGAAACTCTC AAAGTTGATT TTCTTAGCAA GCTACCTGAA ATGCTGAAAA TGTTCGAAGA 2941 TCGTTTATGT CATAAAACAT ATTTAAATGG TGATCATGTA ACCCATCCTG ACTTCATGTT 3001 GTATGACGCT CTTGATGTTG TTTTATACAT GGACCCAATG TGCCTGGATG CGTTCCCAAA 3061 ATTAGTTTGT TTTAAAAAAC GTATTGAAGC TATCCCACAA ATTGATAAGT ACTTGAAATC 3121 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA 3181 TCCTCCAAAA TCGGACAGCA TGCCAGACTC TCTCGAAGTT CTGTTTCAGG GTCCAGCAGG 3241 ATCCatggct atgttccgca gcctggtggc ctcggctcag cagcggcagc cgccggccgg 3301 gccggcgggc ggcgacagcg gcctggaggc gcagtacacc tgccccatct gcctggaggt 3361 ctatcaccgg cccgtggcca tcggcagctg cggccacacg ttctgcgggg agtgtctcca 3421 gccctgcctg caggtgccat ccccgctgtg cccactctgc cgcctgccct tcgaccccaa 3481 gaaggtggac aaggccaccc acgtggagaa gcagctctca tcctacaaag cgccctgtcg 3541 aggctgcaac aaaaaggtga ccctggcaaa gatgagagtg cacatttcgt cctgcctgaa 3601 ggtccaggag cagatggcca actgccccaa gttcgtcccc gtggtgccca catcacagcc 3661 tatccccagc aacatcccca acaggtccac cttcgcctgc ccgtactgtg gtgcccgcaa 3721 cctggaccag caggagctgg tgaagcactg tgtggaaagc caccgcagcg accccaaccg 3781 cgtggtgtgc cccatctgct cggcaatgcc ctggggggac cccagctaca agagcgccaa 3841 cttcctgcag cacctgcttc accgacacaa gttctcctac gacacctttg tggactacag 3901 tattgacgag gaggccgcct tccaggctgc tctggccctg tctctctctg agaacTGAAG 3961 CTTgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag 4021 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa 4081 ggaggaacta tatccggat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.