pLV3-CMV-KLHL33-3×FLAG-P2A-EGF

pLV3-CMV-KLHL33-3×FLAG-P2A-EGFP plasmid

PVT102964

pLV3-CMV-KLHL33-3×FLAG-P2A-EGFP plasmid Information

Plasmid name: pLV3-CMV-KLHL33(human)-3×FLAG-P2A-EGFP
Alias: NM_001365790.2
Gene lenght: 2391
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KLHL33-3×FLAG-P2A-EGFP plasmid Description

pLV3-CMV-KLHL33-3×FLAG-P2A-EGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KLHL33-3×FLAG-P2A-EGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 2041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2461 ccgctagcgc caccatgagc gtctcggaca ccccacatta tccccctgag ctggagagtg 2521 tctctcatcc cgtccagagg gactgccttg gactccttgt gcacgcccag agaacccctt 2581 cctggcctcc ctcccccgac gaggatccca gattgccttc ctttcctctg gaggagcctg 2641 gttccaggcc cctggtccca aggaaccttc cctttccagc cttgtcccta gaggaagaag 2701 aggaagagga agaagatgaa gatgcggcag agcccgagtg gctgcgcagc gaggagcatc 2761 cgagccagtt tttcgccgag gcccagcggc tgcgggagca gagactgttg ctggacgaag 2821 aggtgtcagt cgcggggcgg gtatacgggg tgcatcgggt gatcctggcc gcaatcagca 2881 gcctcttccg agacaggctg ctgggcggcg gaggtccgcg gccccccttc agcctcgagg 2941 tgtccccagg gggctgggag gccgtgctga cctttgccta tgagggggtg ctgggccccg 3001 cctcgcaggg ggatgtgctg gccgcagcag aggcgctggg agcgccccgg gtgaaggctg 3061 ctgcccagca gacatgcgag agggctggaa atgccaggga agatgtaaag aagcccagcc 3121 aggcagagga gctgagggag aacctgcgcg gaatcgagct cctctaccga gagggcgtcg 3181 ggtgtgactt gaagctggag gcaggcggct gccagctgtc ggtgcaccga gccgccctgg 3241 cctgtggcag tgagttcttt ggggccatgc tcctgagcgg gatgagggaa tcccagggca 3301 cagaggtatc tctgcggacg atctccaccc aggacctgcg actcctcgtc tcttttgctt 3361 actccggagt tgtgcgggca aggtggccag ggctactgag agctgcccag gctgctctgc 3421 agtaccagag ctcttcctgc ttggatttgt gtcagaaagg cttggcacgg ggcctcagcc 3481 ctgcccgttg cctggccctg ttccccatgg cggaagcccc tgggttggag aggctctgga 3541 gcaaagcccg tcactacctc ctcacccacc tgcctgctgt agccttgtgt cctgctttcc 3601 cttctttacc agctgcctgc ttggctgagc tcctggatag tgatgagctc catgtgcagg 3661 aggagtttga ggcctttgtg gctgcacggt gttggctggc tgccaacccc gagacccagg 3721 agtcagaggc caaggccctg ctgcgatgtg tccgctttgg ccgcatgtcc accagggagt 3781 tgcggagggt gcgggcagcc gggctacttc cacccctgac cccagatctg ttgcaccagc 3841 tgatggtaga ggctgatgtt ccaggccaag agagacggag ggagcctgac cgggcactgg 3901 tagtgattgg cggggatggg ctcagaccag acatggccct aagacaacca tcccgagcag 3961 tgtggtgggc ccgggccttc cgctgtggcg tgggactggt acgaactgtt gagtgggggc 4021 agctgcctgc cctgcctgcc cccggacgct tccggcatgg ggctgcaagc ctggcaggaa 4081 gtgaactcta tgtgtgtggg ggacaagatt tctacagtca ctccaacacc ctggcttcaa 4141 ctctcaggtg ggagcccagt caagaggact gggaggagat ggctcctttg tcccaggctc 4201 gaagcctttt ctccttggtg gcactggatg gaaaacttta tgccctgggt ggaagacaca 4261 atgatgttgc cctggactct gtggagacct acaaccctga gctcaatgtc tggaggccag 4321 cacctgcact tccagcacca tgttttgccc acgcagctgc gattttggag ggccagttgt 4381 acgtgagcgg tggctgtggt gggactggcc aatacctggc ctcactgatg cactatgacc 4441 ccaaacttga gaagccaggg acgtttctga gccctatggg ggtacctcgg gctggccatg 4501 tcatggctgc attgggtggg aggttgtatg tggcaggtgg gctgggtgag actgaggacc 4561 tgctaagctt tgaggcctat gaactaagga ctgatagctg gactcacctg gcacccctac 4621 cctcccccca tgtgggggct gcaagtgctg tgctgcaggg ggagctactg gtgctcgggg 4681 gctacagtca ccgtacttat gccctctctc accttatcca tgcctactgt cctggcctgg 4741 gccgatggct ctgcctggga actctgccaa ggcctcgggc tgagatgcct gcctgcatcc 4801 tgacactgcc cgctgtgcag cacatagctt tggttcccac cccacaccaa accaaacctg 4861 ctggggtcga cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA 4921 AGGATGACGA TGACAAGGGA AGCGGAGCCA CTAACTTCTC CCTGTTGAAA CAAGCAGGGG 4981 ATGTCGAAGA GAATCCCGGG CCAgaattcG CCACCatggt gagcaagggc gaggagctgt 5041 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 5101 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 5161 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 5221 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 5281 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 5341 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 5401 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 5461 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 5521 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 5581 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 5641 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 5701 ggatcactct cggcatggac gagctgtaca agtaaGGATC Caatcaacct ctggattaca 5761 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 5821 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 5881 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 5941 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 6001 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 6061 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 6121 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 6181 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 6241 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga 6301 gtcggatctc cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg 6361 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 6421 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 6481 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 6541 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 6601 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 6661 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 6721 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 6781 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 6841 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 6901 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 6961 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 7021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7081 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 7381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 7441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 7501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 7561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7861 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 7981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 8101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8161 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 8221 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 8281 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 8341 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 8401 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 8461 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 8521 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 8581 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8641 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8701 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 8761 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8821 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8881 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 8941 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9001 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 9061 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9121 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 9181 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 9241 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 9301 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 9361 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 9421 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 9481 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 9541 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 9601 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.